Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
0JO 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid
GOL Glycerol
OAS O-acetylserine
Code : 5DBE   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/TRANSFERASE INHIBITOR
Title : Crystal structure of O-acetylserine sulfhydrylase from Haemophilus influenzae in complex with pre-reactive O-acetyl serine, alpha-aminoacrylate reaction intermediate and peptide inhibitor at the resolution of 2.25A
Release Data : 2015-09-09
Compound :
mol_id molecule chains synonym
1 Cysteine synthase X CSase,O-acetylserine (thiol)-lyase,OAS-TL,O-acetylserine sulfhydrylase
ec: 2.5.1.47
mol_id molecule chains synonym
2 C-terminal peptide from Serine acetyltransferase A SAT,Serine transacetylase
Source :
mol_id organism_scientific expression_system
1 Haemophilus influenzae KW20  (taxid:71421) ESCHERICHIA COLI BL21(DE3)  (taxid:469008)
strain: KW20
atcc: 51907
gene: cysK, HI_1103
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET28A
mol_id organism_scientific
2 Salmonella typhimurium LT2  (taxid:99287)
synthetic: yes
Authors : Singh, A.K., Kaushik, A., Ekka, M.K., Kumaran, S.
Keywords : AMINO-ACID BIOSYNTHESIS, CYSTEINE BIOSYNTHESIS, TRANSFERASE, PYRIDOXAL PHOSPHATE, TRANSFERASE-TRANSFERASE INHIBITOR COMP, TRANSFERASE-TRANSFERASE INHIBITOR complex
Exp. method : X-RAY DIFFRACTION ( 2.25 Å )
Citation :

Crystal Structure Of O-Acetylserine Sulfhydrylase From Haemophilus Influenzae In Complex With Pre-Reactive O-Acetyl Serine, Alpha-Aminoacrylate Reactionintermediate And Peptide Inhibitor At The Resolution Of 2.25A

Singh, A.K.,Kaushik, A.,Ekka, M.K.  et al.
To Be Published 

Chain : X
UniProt : P45040 (CYSK_HAEIN)
Reaction : Reaction=hydrogen sulfide + O-acetyl-L-serine = acetate + L-cysteine; Xref=Rhea:RHEA:14829, ChEBI:CHEBI:29919, ChEBI:CHEBI:30089, ChEBI:CHEBI:35235, ChEBI:CHEBI:58340; EC=2.5.1.47;
Chain : A
UniProt : P29847 (CYSE_SALTY)
Reaction : Reaction=acetyl-CoA + L-serine = CoA + O-acetyl-L-serine; Xref=Rhea:RHEA:24560, ChEBI:CHEBI:33384, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:58340; EC=2.3.1.30; Evidence={ECO:0000269|PubMed:4977445};