Brand  (β version)

PDB ID: 4HN4

Hetero Atom Contents

color scheme of protein:

hetatm:

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information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
0JO 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid PoSSuM
BCN Bicine PoSSuM
CS Cesium ion PoSSuM
F9F 2-({[4-(trifluoromethoxy)phenyl]sulfonyl}amino)ethyl dihydrogen phosphate PoSSuM
PEG Di(hydroxyethyl)ether PoSSuM

Structure summary

Code : 4HN4   PDBj   RCSB PDB   PDBe
Header : LYASE/LYASE INHIBITOR
Title : Tryptophan synthase in complex with alpha aminoacrylate E(A-A) form and the F9 inhibitor in the alpha site
Release Data : 2013-12-25
Compound :
mol_id molecule chains
1 Tryptophan synthase alpha chain A
ec: 4.2.1.20
mol_id molecule chains
2 Tryptophan synthase beta chain B
ec: 4.2.1.20
Source :
mol_id organism_scientific expression_system
1 Salmonella enterica subsp. enterica serovar Typhimurium  (taxid:90371) Escherichia coli  (taxid:562)
gene: STM1727, trpA, trpA and trpB
expression_system_strain: CB149
expression_system_vector_type: Derivative of pBR322
expression_system_plasmid: pEBA-10
mol_id organism_scientific
2 Salmonella enterica subsp. enterica serovar Typhimurium  (taxid:90371)
Authors : Hilario, E., Niks, D., Dunn, M.F., Mueller, L.J., Fan, L.
Keywords : Lyase, carbon-oxygen lyase, tryptophan biosynthesis, Salmonella, F9F, Allosteric enzyme, Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Pyridoxal phosphate, alpha amino acrylate, LYASE-LYASE INHIBITOR complex
Exp. method : X-RAY DIFFRACTION ( 1.6400 Å )
Citation :

Allostery and substrate channeling in the tryptophan synthase bienzyme complex: evidence for two subunit conformations and four quaternary states.

Niks, D.,Hilario, E.,Dierkers, A.  et al.
(2013)  Biochemistry  52 : 6396 - 6411

PubMed: 23952479
DOI: 10.1021/bi400795e

Reaction

Chain : A
UniProt : P00929 (TRPA_SALTY)
Reaction : Reaction=(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D- glyceraldehyde 3-phosphate + H2O + L-tryptophan; Xref=Rhea:RHEA:10532, ChEBI:CHEBI:15377, ChEBI:CHEBI:33384, ChEBI:CHEBI:57912, ChEBI:CHEBI:58866, ChEBI:CHEBI:59776; EC=4.2.1.20; Evidence={ECO:0000255|HAMAP-Rule:MF_00131};
Chain : B
UniProt : P0A2K1 (TRPB_SALTY)
Reaction : Reaction=(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D- glyceraldehyde 3-phosphate + H2O + L-tryptophan; Xref=Rhea:RHEA:10532, ChEBI:CHEBI:15377, ChEBI:CHEBI:33384, ChEBI:CHEBI:57912, ChEBI:CHEBI:58866, ChEBI:CHEBI:59776; EC=4.2.1.20;