Brand  (β version)

color scheme of protein:

hetatm:

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information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
IM5 2-amino-7-{[(3r,4r)-3-hydroxy-4-(hydroxymethyl)pyrrolidin-1-yl]methyl}-3,5-dihydro-4h-pyrrolo[3,2-D]pyrimidin-4-one
PO4 Phosphate ion
Modified Residues
Code Name Link
FT6 6-fluoro-L-tryptophan
Code : 4EAR   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/TRANSFERASE INHIBITOR
Title : Crystal structure of purine nucleoside phosphorylase (W16Y, W94Y, W178Y, H257W) mutant from human complexed with DADMe-ImmG and phosphate
Release Data : 2013-02-06
Compound :
mol_id molecule chains synonym
1 Purine nucleoside phosphorylase A,B,C PNP, Inosine phosphorylase
ec: 2.4.2.1
mutation: W16Y, W94Y, W178Y, H257W
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:469008)
gene: NP, PNP, X00737.1
expression_system_strain: BL21(DE3)pLysS
expression_system_vector_type: plasmid
expression_system_plasmid: pCRT7/NT-TOPO
Authors : Haapalainen, A.M., Ho, M.C., Suarez, J.J., Almo, S.C., Schramm, V.L.
Keywords : PNP, Immucillin, Purine nucleoside phosphorylase, nucleoside binding, purine base binding, purine-nucleoside phosphorylase activity, drug binding, transferase activity, transferring glycosyl groups, phosphate ion binding, cytosol, 6-FLUORO-L-TRYPTOPHAN, TRANSFERASE-TRANSFERASE INHIBITOR complex
Exp. method : X-RAY DIFFRACTION ( 1.70 Å )
Citation :

Catalytic Site Conformations in Human PNP by (19)F-NMR and Crystallography.

Suarez, J.,Haapalainen, A.M.,Cahill, S.M.  et al.
(2013)  Chem.Biol.  20 : 212 - 222

PubMed: 23438750
DOI: 10.1016/j.chembiol.2013.01.009

Chain : A, B, C
UniProt : P00491 (PNPH_HUMAN)
Reaction : Reaction=a purine D-ribonucleoside + phosphate = a purine nucleobase + alpha-D-ribose 1-phosphate; Xref=Rhea:RHEA:19805, ChEBI:CHEBI:26386, ChEBI:CHEBI:43474, ChEBI:CHEBI:57720, ChEBI:CHEBI:142355; EC=2.4.2.1;