Brand  (β version)

PDB ID: 4E3J

Hetero Atom Contents

color scheme of protein:

hetatm:

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information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
0N4 [({[4-(1h-tetrazol-5-yl)phenyl]sulfonyl}amino)methyl]boronic acid PoSSuM
PO4 Phosphate ion PoSSuM

Structure summary

Code : 4E3J   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/HYDROLASE INHIBITOR
Title : Crystal structure of AmpC beta-lactamase in complex with a designed 4-tetrazolyl benzene sulfonamide boronic acid inhibitor
Release Data : 2012-09-26
Compound :
mol_id molecule chains synonym
1 Beta-lactamase A,B Cephalosporinase
ec: 3.5.2.6
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:83333) Escherichia coli  (taxid:562)
strain: K12
gene: ampA, ampC, b4150, JW4111
expression_system_strain: JM109
expression_system_vector_type: plasmid
expression_system_plasmid: POGO295
Authors : Eidam, O., Shoichet, B.K.
Keywords : AMPC beta-lactamase, class c, hydrolase, cephalosporinase, HYDROLASE-HYDROLASE INHIBITOR complex
Exp. method : X-RAY DIFFRACTION ( 1.7999 Å )
Citation :

Fragment-guided design of subnanomolar beta-lactamase inhibitors active in vivo.

Eidam, O.,Romagnoli, C.,Dalmasso, G.  et al.
(2012)  Proc.Natl.Acad.Sci.USA  109 : 17448 - 17453

PubMed: 23043117
DOI: 10.1073/pnas.1208337109

Reaction

Chain : A, B
UniProt : P00811 (AMPC_ECOLI)
Reaction : Reaction=a beta-lactam + H2O = a substituted beta-amino acid; Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627, ChEBI:CHEBI:140347; EC=3.5.2.6; Evidence={ECO:0000255|PROSITE- ProRule:PRU10102};