Brand  (β version)

PDB ID: 3U5Z

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Ligands
Code Name Style Show Link
08T [[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium PoSSuM
MG Magnesium ion PoSSuM
ADP Adenosine-5'-diphosphate PoSSuM
Non-standard Residues
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MSE Selenomethionine
Glycosylation
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Modification
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Structure summary

Code : 3U5Z   PDBj   RCSB PDB   PDBe
Header : DNA BINDING PROTEIN/DNA
Title : Structure of T4 Bacteriophage clamp loader bound to the T4 clamp, primer-template DNA, and ATP analog
Release Data : 2012-01-04
Compound :
mol_id molecule chains synonym
1 DNA polymerase accessory protein 44 B,C,D,E,L,M,N,O Clamp loader large subunit, Protein Gp44
mol_id molecule chains synonym
2 DNA polymerase accessory protein 62 A,K Clamp loader small subunit, Protein Gp62
mol_id molecule chains synonym
3 DNA polymerase processivity component G,H,F,Q,R,P DNA polymerase accessory protein 45, Gp45
mol_id molecule chains
4 Template DNA strand I,S
mol_id molecule chains
5 Primer DNA strand J,T
Source :
mol_id organism_scientific expression_system
1 Enterobacteria phage T4  (taxid:10665) Escherichia coli  (taxid:562)
gene: 44, gp44
expression_system_strain: T7 Express
mol_id organism_scientific expression_system
2 Enterobacteria phage T4  (taxid:10665) Escherichia coli  (taxid:562)
gene: 62, gp62
expression_system_strain: T7 Express
mol_id organism_scientific expression_system
3 Enterobacteria phage T4  (taxid:10665) Escherichia coli  (taxid:562)
gene: 45, gp45
expression_system_strain: T7 Express
mol_id organism_scientific
4
synthetic: yes
mol_id organism_scientific
5
synthetic: yes
Authors : Kelch, B.A., Makino, D.L., O'Donnell, M., Kuriyan, J.
Keywords : AAA+, ATP hydrolase, clamp loader, sliding clamp, DNA BINDING PROTEIN-DNA complex
Exp. method : X-RAY DIFFRACTION ( 3.500 Å )
Citation :

How a DNA polymerase clamp loader opens a sliding clamp.

Kelch, B.A.,Makino, D.L.,O'Donnell, M.  et al.
(2011)  Science  334 : 1675 - 1680

PubMed: 22194570
DOI: 10.1126/science.1211884

Reaction

Chain : B, C, D, E, L, M, N, O
UniProt : P04526 (LOADL_BPT4)
Reaction : -
Chain : A, K
UniProt : P04527 (LOADS_BPT4)
Reaction : -
Chain : G, H, F, Q, R, P
UniProt : P04525 (CLAMP_BPT4)
Reaction : -