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Ligands
Code Name Link
CNU 5-cyano-uridine-5'-monophosphate
SO4 Sulfate ion
Code : 3EWZ   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : human orotidyl-5'-monophosphate decarboxylase in complex with 5-cyano-UMP
Release Data : 2009-04-07
Compound :
mol_id molecule chains synonym
1 Orotidine-5'-phosphate decarboxylase A,B,C,D OMPdecase
ec: 4.1.1.23
fragment: UNP residues 224-480
Source :
mol_id organism_scientific expression_system
1 Homo sapiens  (taxid:9606) Escherichia coli  (taxid:562)
gene: UMPS
expression_system_strain: Rosetta2(DE3)
expression_system_vector_type: Plasmid
expression_system_plasmid: pETM-30
Authors : Heinrich, D., Diederichsen, U., Rudolph, M.
Keywords : decarboxylase, tim barrel, unusual catalysis, Alternative splicing, Disease mutation, Glycosyltransferase, Lyase, Multifunctional enzyme, Polymorphism, Pyrimidine biosynthesis, Transferase
Exp. method : X-RAY DIFFRACTION ( 1.40 Å )
Citation :

Lys314 is a nucleophile in non-classical reactions of orotidine-5'-monophosphate decarboxylase

Heinrich, D.,Diederichsen, U.,Rudolph, M.G.
(2009)  Chemistry  15 : 6619 - 6625

PubMed: 19472232
DOI: 10.1002/chem.200900397

Chain : A, B, C, D
UniProt : P11172 (UMPS_HUMAN)
Reaction : Reaction=diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D- ribose 1-diphosphate + orotate; Xref=Rhea:RHEA:10380, ChEBI:CHEBI:30839, ChEBI:CHEBI:33019, ChEBI:CHEBI:57538, ChEBI:CHEBI:58017; EC=2.4.2.10;
Reaction=H(+) + orotidine 5'-phosphate = CO2 + UMP; Xref=Rhea:RHEA:11596, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57538, ChEBI:CHEBI:57865; EC=4.1.1.23;