Brand  (β version)

PDB ID: 3EFS

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Ligands
Code Name Style Show Link
ATP Adenosine-5'-triphosphate PoSSuM
BTN Biotin PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
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Glycosylation
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Modification
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Structure summary

Code : 3EFS   PDBj   RCSB PDB   PDBe
Header : LIGASE
Title : Biotin protein ligase from Aquifex aeolicus in complex with biotin and ATP
Release Data : 2009-06-09
Compound :
mol_id molecule chains synonym
1 Biotin [acetyl-CoA-carboxylase] ligase A,B Biotin Protein Ligase
ec: 6.3.4.15
Source :
mol_id organism_scientific expression_system
1 Aquifex aeolicus  (taxid:63363) Escherichia coli BL21(DE3)  (taxid:469008)
expression_system_strain: BL21 (DE3)
expression_system_vector_type: Plasmid
expression_system_plasmid: pET28a
Authors : Tron, C.M., McNae, I.W., Walkinshaw, M.D., Baxter, R.L., Campopiano, D.J.
Keywords : BPL ATP BIOTIN COMPLEX, Ligase
Exp. method : X-RAY DIFFRACTION ( 2.300 Å )
Citation :

Structural and functional studies of the biotin protein ligase from Aquifex aeolicus reveal a critical role for a conserved residue in target specificity.

Tron, C.M.,McNae, I.W.,Nutley, M.  et al.
(2009)  J.Mol.Biol.  387 : 129 - 146

PubMed: 19385043
DOI: 10.1016/j.jmb.2008.12.086

Reaction

Chain : A, B
UniProt : O66837 (O66837_AQUAE)
Reaction: EC: Evidence:
Physiological Direction:
biotin + L-lysyl-[protein] + ATP = N(6)-biotinyl-L-lysyl- [protein] + AMP + diphosphate + H(+) 6.3.4.15 ARBA:ARBA00047846
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