Brand  (β version)

PDB ID: 2VQU

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Ligands
Code Name Style Show Link
EDO 1,2-ethanediol PoSSuM
NOY (2r,3s,4r,5r)-5-(hydroxymethyl)piperidine-2,3,4-triol PoSSuM
BR Bromide ion PoSSuM
CL Chloride ion PoSSuM
Non-standard Residues
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Structure summary

Code : 2VQU   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Structural and biochemical evidence for a boat-like transition state in beta-mannosidases
Release Data : 2008-04-01
Compound :
mol_id molecule chains
1 BETA-MANNOSIDASE A,B
ec: 3.2.1.25
fragment: RESIDUES 26-864
mutation: YES
Source :
mol_id organism_scientific expression_system
1 BACTEROIDES THETAIOTAOMICRON  (taxid:818) ESCHERICHIA COLI  (taxid:511693)
variant: VPI-5482
atcc: 29148
expression_system_strain: BL21
expression_system_plasmid: PET28A
Authors : Tailford, L.E., Offen, W.A., Smith, N.L., Dumon, C., Moreland, C., Gratien, J., Heck, M.P., Stick, R.V., Bleriot, Y., Vasella, A., Gilbert, H.J., Davies, G.J.
Keywords : LINEAR FREE ENERGY RELATIONSHIP, TRANSITION STATE MIMIC, MANNOSIDASE, GLYCOSIDE HYDROLASE, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.90 Å )
Citation :

Structural and Biochemical Evidence for a Boat-Like Transition State in Beta-Mannosidases.

Tailford, L.E.,Offen, W.A.,Smith, N.L.  et al.
(2008)  Nat.Chem.Biol.  4 : 306

PubMed: 18408714
DOI: 10.1038/NCHEMBIO.81

Reaction

Chain : A, B
UniProt : Q8AAK6 (Q8AAK6_BACTN)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. 3.2.1.25 ARBA:ARBA00000829
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