Brand  (β version)

PDB ID: 2PYL

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Ligands
Code Name Style Show Link
EDO 1,2-ethanediol PoSSuM
MG Magnesium ion PoSSuM
TTP Thymidine-5'-triphosphate PoSSuM
Non-standard Residues
Code Name Show
2DA 2',3'-dideoxyadenosine-5'-monophosphate
Glycosylation
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Modification
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Structure summary

Code : 2PYL   PDBj   RCSB PDB   PDBe
Header : REPLICATION, TRANSFERASE/DNA
Title : Phi29 DNA polymerase complexed with primer-template DNA and incoming nucleotide substrates (ternary complex)
Release Data : 2007-07-17
Compound :
mol_id molecule chains
1 5'-d(GACTGCTTAC(2DA))-3' X
mol_id molecule chains
2 5'-d(CTGACGAATGTACA)-3' Y
mol_id molecule chains synonym
3 DNA polymerase A Early protein GP2
ec: 2.7.7.7
mutation: D12A,D66A
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Bacillus phage phi29  (taxid:10756) Escherichia coli  (taxid:562)
gene: 2, gp2
Authors : Berman, A.J., Kamtekar, S., Goodman, J.L., Lazaro, J.M., de Vega, M., Blanco, L., Salas, M., Steitz, T.A.
Keywords : protein-dna complex, REPLICATION, TRANSFERASE-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.20 Å )
Citation :

Structures of phi29 DNA polymerase complexed with substrate: the mechanism of translocation in B-family polymerases

Berman, A.J.,Kamtekar, S.,Goodman, J.L.  et al.
(2007)  Embo J.  26 : 3494 - 3505

PubMed: 17611604
DOI: 10.1038/sj.emboj.7601780

Reaction

Chain : A
UniProt : P03680 (DPOL_BPPH2)
Reaction: EC: Evidence:
Physiological Direction:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.7 PubMed:3863101
-
Cofactor: Evidence: Note:
Mg(2+) ECO:0000269 | PubMed:1310035
ECO:0000269 | PubMed:15608377
ECO:0000269 | PubMed:17611604
ECO:0000269 | PubMed:9784372
-