Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
FLV Flaviolin
HEM Protoporphyrin ix containing Fe
OXY Oxygen molecule
Code : 2D09   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : A Role for Active Site Water Molecules and Hydroxyl Groups of Substrate for Oxygen Activation in Cytochrome P450 158A2
Release Data : 2005-10-25
Compound :
mol_id molecule chains
1 putative cytochrome P450 A
ec: 1.14.-.-
Source :
mol_id organism_scientific expression_system
1 Streptomyces coelicolor  (taxid:100226) Escherichia coli  (taxid:562)
strain: A3(2)
expression_system_strain: HMS174(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET17b
Authors : Zhao, B., Waterman, M.R.
Keywords : streptomyces, Cytochrome P450 oxidoreductase, CYP158A2, proton transfer, dioxygen activation, Oxidoreductase
Exp. method : X-RAY DIFFRACTION ( 1.800 Å )
Citation :

Role of active site water molecules and substrate hydroxyl groups in oxygen activation by cytochrome P450 158A2: a new mechanism of proton transfer

Zhao, B.,Guengerich, F.P.,Voehler, M.  et al.
(2005)  J.Biol.Chem.  280 : 42188 - 42197

PubMed: 16239228
DOI: 10.1074/jbc.M509220200

Binding of two flaviolin substrate molecules, oxidative coupling, and crystal structure of Streptomyces coelicolor A3(2) cytochrome P450 158A2

Zhao, B.,Guengerich, F.P.,Bellamine, A.  et al.
(2005)  J.Biol.Chem.  280 : 11599 - 11607

PubMed: 15659395
DOI: 10.1074/jbc.M410933200

Chain : A
UniProt : Q9FCA6 (C1582_STRCO)
Reaction : Reaction=2 flaviolin + H(+) + O2 + 2 reduced [2Fe-2S]-[ferredoxin] = 3,3'-biflaviolin + 2 H2O + 2 oxidized [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:26031, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:58696, ChEBI:CHEBI:77877; EC=1.14.19.69; Evidence={ECO:0000269|PubMed:15659395, ECO:0000269|PubMed:16239228, ECO:0000269|PubMed:22203090};
Reaction=2 flaviolin + H(+) + O2 + 2 reduced [2Fe-2S]-[ferredoxin] = 3,8'-biflaviolin + 2 H2O + 2 oxidized [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:26035, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:58696, ChEBI:CHEBI:77840; EC=1.14.19.69; Evidence={ECO:0000269|PubMed:15659395, ECO:0000269|PubMed:16239228, ECO:0000269|PubMed:22203090};