Brand  (β version)

PDB ID: 1SL2

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Ligands
Code Name Style Show Link
DAD 2',3'-dideoxyadenosine-5'-triphosphate PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
Code Name Show
2DA 2',3'-dideoxyadenosine-5'-monophosphate
TTD Cis-syn cyclobutane thymine dimer
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 1SL2   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/ELECTRON TRANSPORT/DNA
Title : Ternary 5' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the template and an incoming nucleotide
Release Data : 2004-07-06
Compound :
mol_id molecule chains
1 5'-D(*CP*GP*AP*AP*AP*AP*CP*GP*AP*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*TP*(2DA))-3' P
other_details: DNA primer
mol_id molecule chains
2 5'-D(*CP*CP*CP*(TTD)P*AP*GP*GP*CP*AP*CP*TP*GP*GP*CP*CP*GP*TP*CP*GP*TP*TP*TP*TP*CP*G)-3' T
other_details: DNA template
mol_id molecule chains synonym
3 DNA polymerase A T7 DNA polymerase
ec: 2.7.7.7
mutation: DEL(118-123)
mol_id molecule chains synonym
4 Thioredoxin 1 B TRX1, TRX
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Enterobacteria phage T7  (taxid:10760) Escherichia coli  (taxid:562)
gene: 5
mol_id organism_scientific expression_system
4 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
gene: TRXA, TSNC, FIPA, B3781, C4701, Z5291, ECS4714, STM3915, STMD1.75, STY3639, T3381, SF3854, S3905
Authors : Li, Y., Dutta, S., Doublie, S., Bdour, H.M., Taylor, J.S., Ellenberger, T.
Keywords : DNA polymerase, fidelity, lesion bypass, thymine dimer, open, close, TRANSFERASE-ELECTRON TRANSPORT-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

Nucleotide insertion opposite a cis-syn thymine dimer by a replicative DNA polymerase from bacteriophage T7.

Li, Y.,Dutta, S.,Doublie, S.  et al.
(2004)  Nat.Struct.Mol.Biol.  11 : 784 - 790

PubMed: 15235589
DOI: 10.1038/nsmb792

Reaction

Chain : A
UniProt : P00581 (DPOL_BPT7)
Reaction: EC: Evidence:
Physiological Direction:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.7 HAMAP-Rule:MF_04101, PubMed:24591606
-
Cofactor: Evidence: Note:
Mg(2+) ECO:0000255 | HAMAP-Rule:MF_04101
ECO:0000305 | PubMed:9440688
-
Chain : B
UniProt : P0AA25 (THIO_ECOLI)
Reaction : -