Brand  (β version)

PDB ID: 1LZQ

Hetero Atom Contents

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hetatm:

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information
centroid:
interaction residue:

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Ligands
Code Name Link
0ZQ N-{(3s)-3-[(tert-butoxycarbonyl)amino]-4-phenylbutyl}-L-phenylalanyl-L-alpha-glutamyl-L-phenylalaninamide PoSSuM
BME Beta-mercaptoethanol PoSSuM

Structure summary

Code : 1LZQ   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/HYDROLASE INHIBITOR
Title : Crystal structure of the complex of mutant HIV-1 protease (A71V, V82T, I84V) with an ethylenamine peptidomimetic inhibitor BOC-PHE-PSI[CH2CH2NH]-PHE-GLU-PHE-NH2
Release Data : 2003-04-29
Compound :
mol_id molecule chains synonym
1 PROTEASE RETROPEPSIN A,B HIV-1 PROTEASE
ec: 3.4.23.16
mutation: A71V, V82T, I84V
Source :
mol_id organism_scientific expression_system
1 Human immunodeficiency virus 1  (taxid:11676) Escherichia coli  (taxid:562)
Authors : Skalova, T., Hasek, J., Dohnalek, J., Petrokova, H., Buchtelova, E., Soucek, M., Majer, P., Uhlikova, T., Konvalinka, J.
Keywords : HIV, PEPTIDOMIMETIC, ethylenamine ISOSTERE, HYDROLASE-HYDROLASE INHIBITOR COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.20 Å )
Citation :

An Ethylenamine Inhibitor Binds Tightly to Both Wild Type and Mutant HIV-1 Proteases. Structure and Energy Study

Skalova, T.,Hasek, J.,Dohnalek, J.  et al.
(2003)  J.Med.Chem.  46 : 1636 - 1644

PubMed: 12699382
DOI: 10.1021/jm021079g

Reaction

Chain : A, B
UniProt : P03367 (POL_HV1BR)
Reaction : Reaction=Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.; EC=3.4.23.16; Evidence={ECO:0000255|PROSITE-ProRule:PRU00275};
Reaction=Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.; EC=3.1.26.13;
Reaction=3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.; EC=3.1.13.2;
Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:11130, Rhea:RHEA-COMP:11131, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:83828; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE- ProRule:PRU00405};
Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:11130, Rhea:RHEA-COMP:11131, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:83828; EC=2.7.7.7; Evidence={ECO:0000255|PROSITE- ProRule:PRU00405};