Brand  (β version)

PDB ID: 1E6C

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Ligands
Code Name Style Show Link
PO4 Phosphate ion PoSSuM
CL Chloride ion PoSSuM
MPD (4s)-2-methyl-2,4-pentanediol PoSSuM
MRD (4r)-2-methylpentane-2,4-diol PoSSuM
Non-standard Residues
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Structure summary

Code : 1E6C   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : K15M MUTANT OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI
Release Data : 2001-06-20
Compound :
mol_id molecule chains
1 SHIKIMATE KINASE A,B
ec: 2.7.1.71
mutation: YES
Source :
mol_id organism_scientific expression_system
1 ERWINIA CHRYSANTHEMI  (taxid:556) ESCHERICHIA COLI  (taxid:469008)
gene: AROL
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_vector: T7 RNA POLYMERASE BASED
expression_system_plasmid: PTB361SK
expression_system_gene: AROL
Authors : Maclean, J., Krell, T., Coggins, J.R., Lapthorn, A.J.
Keywords : MUTANT SHIKIMATE KINASE, PHOSPHORYL TRANSFER, ADP, SHIKIMATE PATHWAY, P-LOOP PROTEIN, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 1.8 Å )
Citation :

Biochemical and X-Ray Crystallographic Studies on Shikimate Kinase: The Important Structural Role of the P-Loop Lysine

Krell, T.,Maclean, J.,Boam, D.J.  et al.
(2001)  Protein Sci.  10 : 1137

PubMed: 11369852
DOI: 10.1110/PS.52501

The Three-Dimensional Structure of Shikimate Kinase

Krell, T.,Coggins, J.R.,Lapthorn, A.J.
(1998)  J.Mol.Biol.  278 : 983

PubMed: 9600856
DOI: 10.1006/JMBI.1998.1755

Reaction

Chain : A, B
UniProt : P10880 (AROL_DICCH)
Reaction: EC: Evidence:
Physiological Direction:
shikimate + ATP = 3-phosphoshikimate + ADP + H(+) 2.7.1.71 -
-
Cofactor: Evidence: Note:
Mg(2+) ECO:0000269 | PubMed:9600856
Binds 1 Mg(2+) ion per subunit.