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Ligands
Code Name Style Show Link
CA Calcium ion
Non-standard Residues
Code Name Show
5CM 5-methyl-2'-deoxy-cytidine-5'-monophosphate
Glycosylation
Code Name Emphasize
Modification
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Code : 1BSU   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/DNA
Title : STRUCTURAL AND ENERGETIC ORIGINS OF INDIRECT READOUT IN SITE-SPECIFIC DNA CLEAVAGE BY A RESTRICTION ENDONUCLEASE
Release Data : 1998-09-09
Compound :
mol_id molecule chains
1 DNA (5'-D(P*AP*AP*GP*AP*(5CM)P*IP*TP*CP*TP*T)-3') C
mol_id molecule chains
2 DNA (5'-D(*AP*AP*AP*GP*AP*(5CM)P*IP*TP*CP*TP*T)-3') D
mol_id molecule chains
3 ENDONUCLEASE ECORV (3.1.21.4) A,B
ec: 3.1.21.4
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
Authors : Perona, J.J., Martin, A.M.
Keywords : COMPLEX ENDONUCLEASE ECORV (3.1.21.4)-DNA, HYDROLASE-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.0 Å )
Citation :

Structural and energetic origins of indirect readout in site-specific DNA cleavage by a restriction endonuclease.

Martin, A.M.,Sam, M.D.,Reich, N.O.  et al.
(1999)  Nat.Struct.Biol.  6 : 269 - 277

PubMed: 10074946
DOI: 10.1038/6707

Metal Ion Mediated Substrate-Assisted Catalysis in Type II Restriction Endonucleases

Horton, N.C.,Perona, J.J.
(1998)  Proc.Natl.Acad.Sci.USA  95 : 13489 - 13494

Conformational Transitions and Structural Deformability of EcoRV Endonuclease Revealed by Crystallographic Analysis

Perona, J.J.,Martin, A.M.
(1997)  J.Mol.Biol.  273 : 207

Chain : A, B
UniProt : P04390 (T2E5_ECOLX)
Reaction: EC: Evidence:
Physiological Direction:
Endonucleolytic cleavage of DNA to give specific double- stranded fragments with terminal 5'-phosphates. 3.1.21.4 PubMed:1647200, Ref.2
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