Brand  (β version)

PDB ID: 1AQN

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Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
IPA Isopropyl alcohol PoSSuM
UNX Unknown atom or ion PoSSuM
Non-standard Residues
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Structure summary

Code : 1AQN   PDBj   RCSB PDB   PDBe
Header : SERINE PROTEASE
Title : SUBTILISIN MUTANT 8324
Release Data : 1998-01-14
Compound :
mol_id molecule chains
1 SUBTILISIN 8324 A
ec: 3.4.21.62
mutation: T22C, M50F, S87C, G169A, Q206C, Y217K, N218S
Source :
mol_id organism_scientific expression_system
1 Bacillus amyloliquefaciens  (taxid:1390) Bacillus subtilis  (taxid:1423)
strain: GX8324
cellular_location: SECRETED
expression_system_strain: GX8324
expression_system_plasmid: PGX8324
Authors : Whitlow, M., Howard, A.J., Wood, J.F.
Keywords : HYDROLASE, SERINE PROTEINASE, SERINE PROTEASE
Exp. method : X-RAY DIFFRACTION ( 1.80 Å )
Citation :

Large increases in general stabilityfor subtilisin BPN' through incremental changes in the free energy of unfolding

Muralikrishna, P.,Wickstrom, E.
To be published 

The Engineering of Binding Affinity at Metal Ion Binding Sites for the Stabilization of Proteins: Subtilisin as a Test Case

Pantoliano, M.W.,Whitlow, M.,Wood, J.F.  et al.
(1988)  Biochemistry  27 : 8311

Protein Engineering of Subtilisin Bpn': Enhanced Stabilization Through the Introduction of Two Cysteines to Form a Disulfide Bond

Pantoliano, M.W.,Ladner, R.C.,Bryan, P.N.  et al.
(1987)  Biochemistry  26 : 2077

Proteases of Enhanced Stability: Characterization of a Thermostable Variant of Subtilisin

Bryan, P.N.,Rollence, M.L.,Pantoliano, M.W.  et al.
(1986)  Proteins  1 : 326

Atomic Coordinates for Subtilisin Bpn' (or Novo)

Alden, R.A.,Birktoft, J.J.,Kraut, J.  et al.
(1971)  Biochem.Biophys.Res.Commun.  45 : 337

Reaction

Chain : A
UniProt : P00782 (SUBT_BACAM)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. 3.4.21.62 -
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