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Ligands
Code Name Link
DDU 2'-5'dideoxyuridine
FMT Formic acid
PO4 Phosphate ion
Code : 1AOB   PDBj   RCSB PDB   PDBe
Header : METHYLTRANSFERASE
Title : E. COLI THYMIDYLATE SYNTHASE COMPLEXED WITH DDURD
Release Data : 1998-07-01
Compound :
mol_id molecule chains synonym
1 THYMIDYLATE SYNTHASE A TS, THYMIDYLATE SYNTHETASE
ec: 2.1.1.45
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
cell_line: CHI-2913
gene: THYA
expression_system_cell_line: CHI-2913
expression_system_vector: BLUESCRIPT
expression_system_plasmid: PTHYA-WT
expression_system_gene: THYA
Authors : Stout, T.J., Sage, C.R., Stroud, R.M.
Keywords : TRANSFERASE (METHYLTRANSFERASE), SUBSTRATE MODULES, METHYLTRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.10 Å )
Citation :

The additivity of substrate fragments in enzyme-ligand binding.

Stout, T.J.,Sage, C.R.,Stroud, R.M.
(1998)  Structure  6 : 839 - 848

PubMed: 9687366
DOI: 10.1016/S0969-2126(98)00086-0

Chain : A
UniProt : P0A884 (TYSY_ECOLI)
Reaction : Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8- dihydrofolate + dTMP; Xref=Rhea:RHEA:12104, ChEBI:CHEBI:15636, ChEBI:CHEBI:57451, ChEBI:CHEBI:63528, ChEBI:CHEBI:246422; EC=2.1.1.45; Evidence={ECO:0000255|HAMAP-Rule:MF_00008, ECO:0000269|PubMed:2223754, ECO:0000269|PubMed:3286637, ECO:0000269|PubMed:9826509};