Brand  (β version)

PDB ID: 1AKD

Hetero Atom Contents

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
CAM Camphor PoSSuM
HEM Protoporphyrin ix containing Fe PoSSuM
K Potassium ion PoSSuM

Structure summary

Code : 1AKD   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : CYTOCHROME P450CAM FROM PSEUDOMONAS PUTIDA, COMPLEXED WITH 1S-CAMPHOR
Release Data : 1997-11-19
Compound :
mol_id molecule chains synonym
1 CYTOCHROME P450CAM A CAMPHOR MONOOXYGENASE
ec: 1.14.15.1
other_details: HEME LINKED BY CYS 357, SUBSTRATE (1S)-CAMPHOR BOUND IN TWO CONFORMATIONS, A AND B
Source :
mol_id organism_scientific expression_system
1 Pseudomonas putida  (taxid:303) Escherichia coli  (taxid:562)
Authors : Schlichting, I., Jung, C., Schulze, H.
Keywords : OXIDOREDUCTASE, OXYGENASE, CYTOCHROME P450, MONOOXYGENASE, ELECTRON TRANSPORT
Exp. method : X-RAY DIFFRACTION ( 1.8 Å )
Citation :

Crystal structure of cytochrome P-450cam complexed with the (1S)-camphor enantiomer.

Schlichting, I.,Jung, C.,Schulze, H.
(1997)  FEBS Lett.  415 : 253 - 257

PubMed: 9357977
DOI: 10.1016/S0014-5793(97)01135-6

High-Resolution Crystal Structure of Cytochrome P450Cam

Poulos, T.L.,Finzel, B.C.,Howard, A.J.
(1987)  J.Mol.Biol.  195 : 687

Reaction

Chain : A
UniProt : P00183 (CPXA_PSEPU)
Reaction : Reaction=(1R,4R)-camphor + 2 H(+) + O2 + 2 reduced [2Fe-2S]- [putidaredoxin] = (1R,4R,5R)-5-hydroxycamphor + H2O + 2 oxidized [2Fe-2S]-[putidaredoxin]; Xref=Rhea:RHEA:13525, Rhea:RHEA-COMP:14157, Rhea:RHEA-COMP:14158, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15396, ChEBI:CHEBI:15398, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738; EC=1.14.15.1;