Het-PDB Navi2 (PDB: 1AK9)

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Ligands
Code	Name	Link
CA	Calcium ion	PoSSuM
IPA	Isopropyl alcohol	PoSSuM
NA	Sodium ion	PoSSuM

Non-standard Residues
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Glycosylation
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Download interaction data: 1AK9

Code :

1AK9 PDBj RCSB PDB PDBe

Header :

SERINE PROTEASE

Title :

SUBTILISIN MUTANT 8321

Release Data :

1997-11-12

Compound :

mol_id	molecule	chains
1	SUBTILISIN 8321	A
	ec: 3.4.21.62 mutation: T22C, M50F, S87C, G169A, Y217K, N218S

Source :

mol_id	organism_scientific	expression_system
1	Bacillus amyloliquefaciens (taxid:1390)	Bacillus subtilis (taxid:1423)
	expression_system_strain: GX8321 expression_system_cellular_location: SECRETED expression_system_plasmid: PGX8321

Authors :

Whitlow, M., Howard, A.J., Wood, J.F.

Keywords :

HYDROLASE, SERINE PROTEASE, SPORULATION

Exp. method :

X-RAY DIFFRACTION ( 1.80 Å )

Citation :

Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding.

Pantoliano, M.W.,Whitlow, M.,Wood, J.F. et al.
(1989) Biochemistry 28 : 7205 - 7213

PubMed: 2684274
DOI: 10.1021/bi00444a012

The Engineering of Binding Affinity at Metal Ion Binding Sites for the Stabilization of Proteins: Subtilisin as a Test Case

Pantoliano, M.W.,Whitlow, M.,Wood, J.F. et al.
(1988) Biochemistry 27 : 8311

Protein Engineering of Subtilisin Bpn': Enhanced Stabilization Through the Introduction of Two Cysteines to Form a Disulfide Bond

Pantoliano, M.W.,Ladner, R.C.,Bryan, P.N. et al.
(1987) Biochemistry 26 : 2077

Proteases of Enhanced Stability: Characterization of a Thermostable Variant of Subtilisin

Bryan, P.N.,Rollence, M.L.,Pantoliano, M.W. et al.
(1986) Proteins 1 : 326

Atomic Coordinates for Subtilisin Bpn' (or Novo)

Alden, R.A.,Birktoft, J.J.,Kraut, J. et al.
(1971) Biochem.Biophys.Res.Commun. 45 : 337

Chain :

UniProt :

P00782 (SUBT_BACAM)

Reaction:	EC:	Evidence:
Reaction:	Physiological Direction:	Evidence:
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.	3.4.21.62	-
	-	-

PDB ID: 1AK9

Hetero Atom Contents

Structure summary

Reaction