Brand  (β version)

PDB ID: 1A9X

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Ligands
Code Name Style Show Link
PO4 Phosphate ion PoSSuM
MN Manganese (II) ion PoSSuM
K Potassium ion PoSSuM
CL Chloride ion PoSSuM
ADP Adenosine-5'-diphosphate PoSSuM
ORN L-ornithine PoSSuM
NET Tetraethylammonium ion PoSSuM
Non-standard Residues
Code Name Show
CYG 2-amino-4-(amino-3-oxo-propylsulfanylcarbonyl)-butyric acid
Glycosylation
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Modification
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Structure summary

Code : 1A9X   PDBj   RCSB PDB   PDBe
Header : AMIDOTRANSFERASE
Title : CARBAMOYL PHOSPHATE SYNTHETASE: CAUGHT IN THE ACT OF GLUTAMINE HYDROLYSIS
Release Data : 1998-10-21
Compound :
mol_id molecule chains
1 CARBAMOYL PHOSPHATE SYNTHETASE (LARGE CHAIN) A,C,E,G
mol_id molecule chains
2 CARBAMOYL PHOSPHATE SYNTHETASE (SMALL CHAIN) B,D,F,H
mutation: H353N
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
mol_id organism_scientific expression_system
2 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
Authors : Thoden, J., Holden, H.
Keywords : AMIDOTRANSFERASE, THIOESTER
Exp. method : X-RAY DIFFRACTION ( 1.8 Å )
Citation :

Carbamoyl phosphate synthetase: caught in the act of glutamine hydrolysis.

Thoden, J.B.,Miran, S.G.,Phillips, J.C.  et al.
(1998)  Biochemistry  37 : 8825 - 8831

PubMed: 9636022
DOI: 10.1021/bi9807761

Reaction

Chain : A, C, E, G
UniProt : P00968 (CARB_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+) 6.3.5.5 HAMAP-Rule:MF_01210, PubMed:4944634
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[Carbamoyl phosphate synthase large chain]
hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+) 		6.3.4.16 HAMAP-Rule:MF_01210, PubMed:4944634
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Cofactor: Evidence: Note:
Mn(2+) ECO:0000255 | HAMAP-Rule:MF_01210
ECO:0000255 | HAMAP-Rule:MF_01210
ECO:0000269 | PubMed:10428826
Binds 4 Mg(2+) or Mn(2+) ions per subunit.
Chain : B, D, F, H
UniProt : P00907 (CARA_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+) 6.3.5.5 HAMAP-Rule:MF_01209, PubMed:4944634
-
[Carbamoyl phosphate synthase small chain]
L-glutamine + H2O = L-glutamate + NH4(+) 		- HAMAP-Rule:MF_01209, PubMed:4944634
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