Brand  (β version)

PDB ID: 1A7T

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Ligands
Code Name Style Show Link
MES 2-(N-morpholino)-ethanesulfonic acid PoSSuM
NA Sodium ion PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1A7T   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : METALLO-BETA-LACTAMASE WITH MES
Release Data : 1998-06-17
Compound :
mol_id molecule chains synonym
1 METALLO-BETA-LACTAMASE A,B CLASS B BETA-LACTAMASE
ec: 3.5.2.6
mutation: A171T, D208N
Source :
mol_id organism_scientific expression_system
1 Bacteroides fragilis  (taxid:817) Escherichia coli  (taxid:562)
strain: TAL3636
variant: CLINICAL ISOLATE
gene: CCRA
Authors : Fitzgerald, P.M.D., Wu, J.K., Toney, J.H.
Keywords : HYDROLASE (BETA-LACTAMASE), METALLO BETA-LACTAMASE, ZINC, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.85 Å )
Citation :

Unanticipated inhibition of the metallo-beta-lactamase from Bacteroides fragilis by 4-morpholineethanesulfonic acid (MES): a crystallographic study at 1.85-A resolution.

Fitzgerald, P.M.,Wu, J.K.,Toney, J.H.
(1998)  Biochemistry  37 : 6791 - 6800

PubMed: 9578564
DOI: 10.1021/bi9730339

High-Yield Expression, Purification, and Characterization of Active, Soluble Bacteroides Fragilis Metallo-Beta-Lactamase, Ccra

Toney, J.H.,Wu, J.K.,Overbye, K.M.  et al.
(1997)  Protein Expr.Purif.  9 : 355

Reaction

Chain : A, B
UniProt : P25910 (BLAB_BACFG)
Reaction: EC: Evidence:
Physiological Direction:
a beta-lactam + H2O = a substituted beta-amino acid 3.5.2.6 PubMed:9712862
-
Cofactor: Evidence: Note:
Zn(2+) ECO:0000269 | PubMed:10210203
ECO:0000269 | PubMed:12019104
ECO:0000269 | PubMed:8805566
ECO:0000269 | PubMed:9416622
ECO:0000269 | PubMed:9545432
ECO:0000269 | PubMed:9578564
ECO:0000269 | PubMed:9712862
ECO:0000269 | PubMed:9761816
Binds 2 Zn(2+) ions per subunit. It can also bind Cd(2+) and Co(2+) to a lesser extent (PubMed:9416622).