Brand  (β version)

PDB ID: 1A05

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Ligands
Code Name Style Show Link
IPM 3-isopropylmalic acid PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1A05   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : CRYSTAL STRUCTURE OF THE COMPLEX OF 3-ISOPROPYLMALATE DEHYDROGENASE FROM THIOBACILLUS FERROOXIDANS WITH 3-ISOPROPYLMALATE
Release Data : 1998-06-17
Compound :
mol_id molecule chains synonym
1 3-ISOPROPYLMALATE DEHYDROGENASE A,B IPMDH, IMDH
ec: 1.1.1.85
Source :
mol_id organism_scientific expression_system
1 Acidithiobacillus ferrooxidans  (taxid:920) Escherichia coli  (taxid:562)
strain: AP19-3
gene: LEUB
expression_system_strain: JA221
expression_system_plasmid: PKKLEUB1
Authors : Imada, K., Inagaki, K., Matsunami, H., Kawaguchi, H., Tanaka, H., Tanaka, N., Namba, K.
Keywords : OXIDOREDUCTASE, DECARBOXYLATING DEHYDROGENASE, LEUCINE BIOSYNTHESIS
Exp. method : X-RAY DIFFRACTION ( 2.00 Å )
Citation :

Structure of 3-isopropylmalate dehydrogenase in complex with 3-isopropylmalate at 2.0 A resolution: the role of Glu88 in the unique substrate-recognition mechanism.

Imada, K.,Inagaki, K.,Matsunami, H.  et al.
(1998)  Structure  6 : 971 - 982

PubMed: 9739088
DOI: 10.1016/S0969-2126(98)00099-9

Overproduction and Substrate Specificity of 3-Isopropylmalate Dehydrogenase from Thiobacillus Ferrooxidans

Matsunami, H.,Kawaguchi, H.,Inagaki, K.  et al.
(1998)  Biosci.Biotechnol.Biochem.  62 : 372

3-Isopropylmalate Dehydrogenase from Chemolithoautotroph Thiobacillus Ferrooxidans: DNA Sequence, Enzyme Purification, and Characterization

Kawaguchi, H.,Inagaki, K.,Kuwata, Y.  et al.
(1993)  J.Biochem.(Tokyo)  114 : 370

Cloning and Expression of the Thiobacillus Ferrooxidans 3-Isopropylmalate Dehydrogenase Gene in Escherichia Coli

Inagaki, K.,Kawaguchi, H.,Kuwata, Y.  et al.
(1990)  J.Ferment.Bioeng.  70 : 71

Reaction

Chain : A, B
UniProt : Q56268 (LEU3_ACIFR)
Reaction: EC: Evidence:
Physiological Direction:
(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH 1.1.1.85 HAMAP- Rule:MF_01033, PubMed:8282728
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