Brand  (β version)

color scheme of protein:

hetatm:

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information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
NA Sodium ion
SO4 Sulfate ion
Modified Residues
Code Name Link
AS 2-deoxy-adenosine -5'-thio-monophosphate
GS Guanosine-5'-thio-monophosphate
OKN 5'-methyl-2'-deoxycytidine-5'-phosphorothioate
OKQ 2'-O-methylcytidine-5'-phosphorothioate
OKT 2'-O-methyluridine-5'-phosphorothioate
PST Thymidine-5'-thiophosphate
RFJ 2'-O-methyl-5'-O-thiophosphonoguanosine
Code : 6YCS
Header : TRANSCRIPTION
Title : Human Transcription Cofactor PC4 DNA-binding domain in complex with full phosphorothioate 5-10-5 2'-O-methyl DNA gapmer antisense oligonucleotide.
Release Data : 2020-04-29
Compound :
mol_id molecule chains synonym
1 PC4 protein A,B,C,D SUB1 homolog (S. cerevisiae),isoform CRA_a,cDNA,FLJ92014,highly similar to Homo sapiens SUB1 homolog (S. cerevisiae) (SUB1),mRNA
mol_id molecule chains
2 DNA (5'-D(P*(OKQ))-D(P*(OKT))-R(P*(RFJ))-D(*(OKQ)P*(OKT)P*(AS)P*(GS)P*(OKN)P*(OKN)P*(PST)P*(OKN)P*(PST)P*(GS)P*(GS)P*(AS)P*(OKT)P*(OKT))-3') E,F
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli BL21(DE3)  (taxid:469008)
gene: PC4, SUB1, hCG_1781938
expression_system_vector_type: plasmid
expression_system_plasmid: pET15b
mol_id organism_scientific
2 Synthetic construct  (taxid:32630)
synthetic: yes
Authors : Hyjek-Skladanowska, M., Vickers, T.A., Napiorkowska, A., Anderson, B., Tanowitz, M., Crooke, S.T., Liang, X., Seth, P.P., Nowotny, M.
Keywords : DNA binding, phosphorothioate DNA, 2'-O-methyl, ASO, TRANSCRIPTION
Exp. method : X-RAY DIFFRACTION ( 3.05 Å )
Citation :

Origins of the Increased Affinity of Phosphorothioate-Modified Therapeutic Nucleic Acids for Proteins.

Hyjek-Skladanowska, M.,Vickers, T.A.,Napiorkowska, A.  et al.
(2020)  J.Am.Chem.Soc.  142 : 7456 - 7468

PubMed: 32202774
DOI: 10.1021/jacs.9b13524

Chain : A, B, C, D
UniProt : Q6IBA2 (No found in current UniProtKB/SwissProt)
Reaction : -