Brand  (β version)

PDB ID: 5BTR

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Ligands
Code Name Style Show Link
ZN Zinc ion PoSSuM
STL Resveratrol PoSSuM
Non-standard Residues
Code Name Show
FDL N~6~-acetyl-N-(4-methyl-2-oxo-2h-chromen-7-yl)-L-lysinamide
Glycosylation
Code Name Emphasize
Modification
Code Name Show
ACE Acetyl group

Structure summary

Code : 5BTR   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/SUBSTRATE
Title : Crystal structure of SIRT1 in complex with resveratrol and an AMC-containing peptide
Release Data : 2015-07-08
Compound :
mol_id molecule chains synonym
1 NAD-dependent protein deacetylase sirtuin-1 A,B,C hSIRT1,Regulatory protein SIR2 homolog 1,SIR2-like protein 1,hSIR2
ec: 3.5.1.-
fragment: UNP residues 143-512 and 641-665
mutation: C253S, C268S, C501S, C502S
mol_id molecule chains
2 AMC-containing peptide D,E,F
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli BL21(DE3)  (taxid:469008)
gene: SIRT1, SIR2L1
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET-28a smt3
mol_id organism_scientific
2 Synthetic  (taxid:32630)
synthetic: yes
Authors : Cao, D., Wang, M., Qiu, X., Liu, D., Jiang, H., Yang, N., Xu, R.M.
Keywords : Deacetylase, Human Sirtuin 1, N-terminal domain, Catalytic domain, C-terminal domain, Resveratrol, Substrate, HYDROLASE-SUBSTRATE complex
Exp. method : X-RAY DIFFRACTION ( 3.20 Å )
Citation :

Structural basis for allosteric, substrate-dependent stimulation of SIRT1 activity by resveratrol

Cao, D.,Wang, M.,Qiu, X.  et al.
(2015)  Genes Dev.  29 : 1316 - 1325

PubMed: 26109052
DOI: 10.1101/gad.265462.115

Reaction

Chain : A, B, C
UniProt : Q96EB6 (SIR1_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
H2O + N(6)-acetyl-L-lysyl-[protein] + NAD(+) = 2''-O-acetyl- ADP-D-ribose + L-lysyl-[protein] + nicotinamide 2.3.1.286 PROSITE-ProRule:PRU00236, PubMed:12006491, PubMed:29765047, PubMed:30409912, PubMed:32034146, PubMed:32538779
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H2O + N(6)-propanoyl-L-lysyl-[protein] + NAD(+) = 3''-O- propanoyl-ADP-D-ribose + L-lysyl-[protein] + nicotinamide - UniProtKB:Q923E4
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H2O + N(6)-(2E)-butenoyl-L-lysyl-[protein] + NAD(+) = 2''-O- (2E)-but-2-enoyl-ADP-D-ribose + L-lysyl-[protein] + nicotinamide - PubMed:28497810
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