Brand  (β version)

PDB ID: 3KKT

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Ligands
Code Name Style Show Link
ZN Zinc ion PoSSuM
0CP 5-{3-[(1s,2s,4r)-bicyclo[2.2.1]hept-2-yloxy]-4-methoxyphenyl}tetrahydropyrimidin-2(1h)-one PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
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Structure summary

Code : 3KKT   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Crystal structure of human PDE4b with 5-[3-[(1S,2S,4R)-Bicyclo[2.2.1]hept-2-yloxy]-4-methoxyp henyl]tetrahydro-2(1H)-pyrimidinone reveals ordering of the C-terminal helix residues 502-509.
Release Data : 2009-11-24
Compound :
mol_id molecule chains synonym
1 cAMP-specific 3',5'-cyclic phosphodiesterase 4B A,B DPDE4, PDE32
ec: 3.1.4.17
fragment: catalytic domain
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: DPDE4, PDE4B
expression_system_strain: ArcticExpress RP (DE3)
expression_system_vector_type: Plasmid
expression_system_plasmid: pET28a
Authors : Cheng, R.K.Y., Crawley, L., Barker, J., Whittaker, M.
Keywords : PHOSPHODIESTERASE, ALTERNATIVE SPLICING, HYDROLASE, Phosphoprotein
Exp. method : X-RAY DIFFRACTION ( 2.48 Å )
Citation :

Crystal structure of human PDE4b with 5-[3-[(1S,2S,4R)-Bicyclo[2.2.1]hept-2-yloxy]-4-methoxyp henyl]tetrahydro-2(1H)-pyrimidinone reveals ordering of the C-terminal helix residues 502-509.

Cheng, R.K.Y.,Crawley, L.,Barker, J.  et al.
To be Published 

Reaction

Chain : A, B
UniProt : Q07343 (PDE4B_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
3',5'-cyclic AMP + H2O = AMP + H(+) 3.1.4.53 PubMed:15260978, PubMed:17519386, PubMed:8392015, PubMed:9371714
left-to-right
Cofactor: Evidence: Note:
Zn(2+) ECO:0000269 | PubMed:10846163
ECO:0000269 | PubMed:15003452
Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions.
Mn(2+) ECO:0000269 | PubMed:10846163
ECO:0000269 | PubMed:15003452
ECO:0000269 | PubMed:15003452
Binds 2 divalent metal cations per subunit. Site 2 has a preference for magnesium and/or manganese ions.