Brand  (β version)

PDB ID: 3K4S

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Ligands
Code Name Style Show Link
0MO (4r)-4-(3-butoxy-4-methoxybenzyl)imidazolidin-2-one PoSSuM
MG Magnesium ion PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3K4S   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : The structure of the catalytic domain of human PDE4d with 4-(3-Butoxy-4-methoxyphenyl)methyl-2-imidazolidone
Release Data : 2009-10-20
Compound :
mol_id molecule chains synonym
1 cAMP-specific 3',5'-cyclic phosphodiesterase 4D A DPDE3, PDE43
ec: 3.1.4.17
fragment: catalytic domain, residues 388-654
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: PDE4D, DPDE3
expression_system_strain: Arctic Express RP (DE3)
expression_system_vector_type: Plasmid
expression_system_plasmid: pET28a
Authors : Crawley, L., Cheng, R.K.Y., Wood, M., Barker, J., Felicetti, B., Whittaker, M.
Keywords : nucleotide binding, phosphodiesterase, Alternative splicing, cAMP, Cytoplasm, Cytoskeleton, Hydrolase, Membrane, Metal-binding, Phosphoprotein
Exp. method : X-RAY DIFFRACTION ( 2.05 Å )
Citation :

The structure of the catalytic domain of human PDE4d with 4-(3-Butoxy-4-methoxyphenyl)methyl-2-imidazolidone

Crawley, L.,Cheng, R.K.Y.,Wood, M.  et al.
To be Published 

Reaction

Chain : A
UniProt : Q08499 (PDE4D_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
3',5'-cyclic AMP + H2O = AMP + H(+) 3.1.4.53 PubMed:9371713
left-to-right
Cofactor: Evidence: Note:
Zn(2+) ECO:0000269 | PubMed:14609333
ECO:0000269 | PubMed:15260978
ECO:0000269 | PubMed:15576036
ECO:0000269 | PubMed:17582435
Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions.
Mn(2+) ECO:0000269 | PubMed:15260978
ECO:0000269 | PubMed:15576036
ECO:0000250 | UniProtKB:Q07343
Binds 2 divalent metal cations per subunit (PubMed:15260978, PubMed:15576036). Site 2 has a preference for magnesium and/or manganese ions (By similarity).