Brand  (β version)

color scheme of protein:

hetatm:
centroid:

chain: Hide other chain(s)

Ligands
Code Name Link
H59 N-{3-[(5s)-5-(1,1-dimethylpropyl)-1-(4-fluoro-3-methylbenzyl)-4-hydroxy-2-oxo-2,5-dihydro-1h-pyrrol-3-yl]-1,1-dioxido-4h-1,4-benzothiazin-7-yl}methanesulfonamide
Code : 3H59
Header : TRANSFERASE
Title : Hepatitis C virus polymerase NS5B with thiazine inhibitor 2
Release Data : 2009-09-08
Compound :
mol_id molecule chains synonym
1 RNA-directed RNA polymerase A,B NS5B, p68
ec: 2.7.7.48
fragment: sequence database residues 2421-2989
Source :
mol_id organism_scientific expression_system
1 Hepatitis C virus  (taxid:11105) Escherichia coli  (taxid:562)
strain: BK
gene: NS5B
Authors : Harris, S.F., Ghate, M.
Keywords : HCV, HEPATITIS, NS5B, TRANSFERASE RNA-DEPENDENT RNA POLYMERASE, Acetylation, Apoptosis, ATP-binding, Capsid protein, Cell membrane, Cytoplasm, Disulfide bond, Endoplasmic reticulum, Envelope protein, Fusion protein, Glycoprotein, Helicase, Host-virus interaction, Hydrolase, Interferon antiviral system evasion, Lipid droplet, Lipoprotein, Membrane, Metal-binding, Mitochondrion, Multifunctional enzyme, Nucleotide-binding, Nucleotidyltransferase, Nucleus, Oncogene, Palmitate, Phosphoprotein, Protease, Ribonucleoprotein, RNA replication, RNA-binding, RNA-directed RNA polymerase, Secreted, Serine protease, SH3-binding, Thiol protease, Transcription, Transcription regulation, Transferase, Transmembrane, Ubl conjugation, Viral nucleoprotein, Virion, Zinc
Exp. method : X-RAY DIFFRACTION ( 2.10 Å )
Citation :

Non-nucleoside inhibitors of HCV polymerase NS5B. Part 3: synthesis and optimization studies of benzothiazine-substituted tetramic acids

de Vicente, J.,Hendricks, R.T.,Smith, D.B.  et al.
(2009)  Bioorg.Med.Chem.Lett.  19 : 5648 - 5651

PubMed: 19700319
DOI: 10.1016/j.bmcl.2009.08.023

Chain : A, B
UniProt : P26663 (POLG_HCVBK)
Reaction : Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
NTP + H(2)O = NDP + phosphate.
ATP + H(2)O = ADP + phosphate.