Brand  (β version)

PDB ID: 3VQB

Hetero Atom Contents

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Code Name Style Show Link
CD Cadmium ion PoSSuM
FBG 6-fluoro-4h-1,3-benzodioxine-8-carboxylic acid PoSSuM
SO4 Sulfate ion PoSSuM

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Glycosylation
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Structure summary

Code : 3VQB   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/TRANSFERASE INHIBITOR
Title : HIV-1 IN core domain in complex with 6-fluoro-4H-1,3-benzodioxine-8-carboxylic acid
Release Data : 2013-01-30
Compound :
mol_id molecule chains
1 POL polyprotein A,B
fragment: integrase core domain, UNP residues 770-927
mutation: C56S, W131D, F139D, F185H
Source :
mol_id organism_scientific expression_system
1 Human immunodeficiency virus 1  (taxid:11676) Escherichia coli  (taxid:562)
strain: NL43
gene: pol
expression_system_strain: BL21(DE3)
expression_system_vector_type: Plasmid
expression_system_plasmid: PeT23b+
Authors : Wielens, J., Chalmers, D.K., Parker, M.W., Scanlon, M.J.
Keywords : RNaseH, DNA binding, DNA cleavage, DNA integration, TRANSFERASE-TRANSFERASE INHIBITOR complex
Exp. method : X-RAY DIFFRACTION ( 2.10 Å )
Citation :

Parallel screening of low molecular weight fragment libraries: do differences in methodology affect hit identification?

Wielens, J.,Headey, S.J.,Rhodes, D.I.  et al.
(2013)  J Biomol Screen  18 : 147 - 159

PubMed: 23139382
DOI: 10.1177/1087057112465979

Reaction

Chain : A, B
UniProt : Q72498 (Q72498_HV1)
Reaction: EC: Evidence:
Physiological Direction:
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid. 3.1.13.2 ARBA:ARBA00000379
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Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus. 3.1.26.13 ARBA:ARBA00023415
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Cofactor: Evidence: Note:
Mg(2+) ECO:0000256 | ARBA:ARBA00001946
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