Brand  (β version)

PDB ID: 2H2Q

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Ligands
Code Name Style Show Link
NAP Nadp nicotinamide-adenine-dinucleotide phosphate PoSSuM
DU 2'-deoxyuridine-5'-monophosphate PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 2H2Q   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE, TRANSFERASE
Title : Crystal structure of Trypanosoma cruzi Dihydrofolate Reductase-Thymidylate synthase
Release Data : 2008-04-08
Compound :
mol_id molecule chains
1 Bifunctional dihydrofolate reductase-thymidylate synthase A,B
ec: 1.5.1.3, 2.1.1.45
Source :
mol_id organism_scientific expression_system
1 Trypanosoma cruzi  (taxid:5693) Escherichia coli  (taxid:562)
strain: Y
expression_system_strain: Rosetta(DE3)pLysS
expression_system_vector_type: plasmid
expression_system_plasmid: pET15b
Authors : Senkovich, O., Schormann, N., Chattopadhyay, D.
Keywords : Bifunctional enzyme in complex with NADP and dUMP, OXIDOREDUCTASE, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.40 Å )
Citation :

Structure-based approach to pharmacophore identification, in silico screening, and three-dimensional quantitative structure-activity relationship studies for inhibitors of Trypanosoma cruzi dihydrofolate reductase function.

Schormann, N.,Senkovich, O.,Walker, K.  et al.
(2008)  Proteins  73 : 889 - 901

PubMed: 18536013
DOI: 10.1002/prot.22115

Reaction

Chain : A, B
UniProt : Q27793 (DRTS_TRYCR)
Reaction: EC: Evidence:
Physiological Direction:
(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH + H(+) 1.5.1.3 -
-
dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8- dihydrofolate + dTMP 2.1.1.45 -
-