Brand  (β version)

PDB ID: 2NNH

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Ligands
Code Name Style Show Link
SO4 Sulfate ion PoSSuM
HEM Protoporphyrin ix containing Fe PoSSuM
9CR (9cis)-retinoic acid PoSSuM
PLM Palmitic acid PoSSuM
Non-standard Residues
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Structure summary

Code : 2NNH   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE,ELECTRON TRANSPORT
Title : CYP2C8dH complexed with 2 molecules of 9-cis retinoic acid
Release Data : 2007-10-23
Compound :
mol_id molecule chains synonym
1 Cytochrome P450 2C8 A,B CYPIIC8; P450 form 1; P450 MP-12/MP-20; P450 IIC2; S-mephenytoin 4-hydroxylase
ec: 1.14.14.1
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: CYP2C8
expression_system_vector_type: Plasmid
expression_system_plasmid: pCW
Authors : Schoch, G.A., Yano, J.K., Stout, C.D., Johnson, E.F.
Keywords : CYP2C8; Human P450 2C8; monooxygenases; substrate complex; palmitic acid; 9-cis retinoic acid; panretin, OXIDOREDUCTASE, ELECTRON TRANSPORT
Exp. method : X-RAY DIFFRACTION ( 2.60 Å )
Citation :

Determinants of cytochrome P450 2C8 substrate binding: structures of complexes with montelukast, troglitazone, felodipine, and 9-cis-retinoic acid.

Schoch, G.A.,Yano, J.K.,Sansen, S.  et al.
(2008)  J.Biol.Chem.  283 : 17227 - 17237

PubMed: 18413310
DOI: 10.1074/jbc.M802180200

Reaction

Chain : A, B
UniProt : P10632 (CP2C8_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
an organic molecule + O2 + reduced [NADPH--hemoprotein reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] 1.14.14.1 PubMed:11093772, PubMed:14559847
left-to-right PubMed:11093772
(5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 + reduced [NADPH-- hemoprotein reductase] = (11R,12S)-epoxy-(5Z,8Z,14Z)-eicosatrienoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:7574697, PubMed:15766564
left-to-right PubMed:7574697
(5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 + reduced [NADPH-- hemoprotein reductase] = (11S,12R)-epoxy-(5Z,8Z,14Z)-eicosatrienoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:7574697, PubMed:15766564
left-to-right PubMed:7574697
(5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 + reduced [NADPH-- hemoprotein reductase] = (14R,15S)-epoxy-(5Z,8Z,11Z)-eicosatrienoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:19965576, PubMed:7574697
left-to-right PubMed:19965576, PubMed:7574697
(5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 + reduced [NADPH-- hemoprotein reductase] = (14S,15R)-epoxy-(5Z,8Z,11Z)-eicosatrienoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:19965576, PubMed:7574697
left-to-right PubMed:19965576
(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + O2 + reduced [NADPH-- hemoprotein reductase] = 11,12-epoxy-(5Z,8Z,14Z,17Z)- eicosatetraenoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:15766564
left-to-right
(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + O2 + reduced [NADPH-- hemoprotein reductase] = 14,15-epoxy-(5Z,8Z,11Z,17Z)- eicosatetraenoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:15766564
left-to-right
(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + O2 + reduced [NADPH-- hemoprotein reductase] = (17R,18S)-epoxy-(5Z,8Z,11Z,14Z)- eicosatetraenoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:19965576
left-to-right
(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + O2 + reduced [NADPH-- hemoprotein reductase] = (17S,18R)-epoxy-(5Z,8Z,11Z,14Z)- eicosatetraenoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:19965576
left-to-right
(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 + reduced [NADPH--hemoprotein reductase] = (19R,20S)-epoxy-(4Z,7Z,10Z,13Z,16Z)- docosapentaenoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:19965576
left-to-right
(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 + reduced [NADPH--hemoprotein reductase] = (19S,20R)-epoxy-(4Z,7Z,10Z,13Z,16Z)- docosapentaenoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:19965576
left-to-right
all-trans-retinoate + O2 + reduced [NADPH--hemoprotein reductase] = all-trans-4-hydroxyretinoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:11093772
left-to-right
17beta-estradiol + O2 + reduced [NADPH--hemoprotein reductase] = 16alpha,17beta-estriol + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:14559847
left-to-right
estrone + O2 + reduced [NADPH--hemoprotein reductase] = 16alpha-hydroxyestrone + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:14559847
left-to-right