Brand  (β version)

PDB ID: 2BEL

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Ligands
Code Name Style Show Link
NAP Nadp nicotinamide-adenine-dinucleotide phosphate PoSSuM
CBO Carbenoxolone PoSSuM
CL Chloride ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 2BEL   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Structure of human 11-beta-hydroxysteroid dehydrogenase in complex with NADP and carbenoxolone
Release Data : 2004-12-06
Compound :
mol_id molecule chains synonym
1 CORTICOSTEROID 11-BETA-DEHYDROGENASE ISOZYME 1 A,B,C,D HUMAN 11-BETA-HYDROXYSTEROID DEHYDROGENASE TYPE 1,11-DH, 11-BETA-HSD1
ec: 1.1.1.146
fragment: RESIDUES 26-284
Source :
mol_id organism_scientific organism_common expression_system
1 HOMO SAPIENS  (taxid:9606) HUMAN ESCHERICHIA COLI  (taxid:469008)
expression_system_strain: BL21(DE3)
expression_system_plasmid: P11
Authors : Kavanagh, K., Wu, X., Svensson, S., Elleby, B., von Delft, F., Debreczeni, J.E., Sharma, S., Bray, J., Edwards, A., Arrowsmith, C., Sundstrom, M., Abrahmsen, L., Oppermann, U.
Keywords : OXIDOREDUCTASE, GLUCOCORTICOID ACTIVATION, DRUG TARGET, INHIBITOR, SHORT-CHAIN DEHYDROGENASE/REDUCTASE, HORMONE METABOLISM, MICROSOME, NADP, STEROID METABOLISM
Exp. method : X-RAY DIFFRACTION ( 2.11 Å )
Citation :

The High Resolution Structures of Human, Murine and Guinea Pig 11-Beta-Hydroxysteroid Dehydrogenase Type 1 Reveal Critical Differences in Active Site Architecture

Wu, X.,Kavanagh, K.,Svensson, S.  et al.
To be Published 

Reaction

Chain : A, B, C, D
UniProt : P28845 (DHI1_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
an 11beta-hydroxysteroid + NADP(+) = an 11-oxosteroid + H(+) + NADPH 1.1.1.146 PubMed:10497248, PubMed:11481269, PubMed:12460758, PubMed:14973125, PubMed:15152005, PubMed:15280030, PubMed:17070044, PubMed:17593962, PubMed:17919905, PubMed:21453287, PubMed:27927697, PubMed:30902677, PubMed:8319583
right-to-left PubMed:10497248, PubMed:11481269, PubMed:12460758, PubMed:14973125, PubMed:15152005, PubMed:21453287, PubMed:30902677, PubMed:8319583, PubMed:15280030, PubMed:17593962, PubMed:27927697
cortisone + H(+) + NADPH = cortisol + NADP(+) - PubMed:11481269, PubMed:12460758, PubMed:15152005, PubMed:15280030, PubMed:17593962, PubMed:21453287, PubMed:27927697, PubMed:30902677, PubMed:8319583
right-to-left PubMed:12460758, PubMed:15152005, PubMed:21453287, PubMed:30902677, PubMed:8319583, PubMed:15280030, PubMed:17593962, PubMed:27927697
corticosterone + NADP(+) = 11-dehydrocorticosterone + H(+) + NADPH - PubMed:10497248, PubMed:14973125
right-to-left
a 7beta-hydroxysteroid + NADP(+) = a 7-oxosteroid + H(+) + NADPH 1.1.1.201 PubMed:14973125, PubMed:15095019, PubMed:15152005, PubMed:17593962, PubMed:21453287
right-to-left PubMed:14973125, PubMed:15095019, PubMed:15152005, PubMed:21453287, PubMed:17593962
7-oxocholesterol + H(+) + NADPH = 7beta-hydroxycholesterol + NADP(+) - PubMed:14973125, PubMed:15095019, PubMed:15152005
left-to-right
chenodeoxycholate + NADP(+) = 7-oxolithocholate + H(+) + NADPH - PubMed:21453287
right-to-left
7-oxolithocholate + H(+) + NADPH = NADP(+) + ursodeoxycholate - PubMed:21453287
left-to-right
glycochenodeoxycholate + NADP(+) = 7-oxoglycolithocholate + H(+) + NADPH - PubMed:21453287
right-to-left
NADP(+) + taurochenodeoxycholate = 7-oxotaurolithocholate + H(+) + NADPH - PubMed:21453287
right-to-left
NADP(+) + tauroursodeoxycholate = 7-oxotaurolithocholate + H(+) + NADPH - PubMed:21453287
right-to-left
glycoursodeoxycholate + NADP(+) = 7-oxoglycolithocholate + H(+) + NADPH - PubMed:21453287
right-to-left
7-oxopregnenolone + H(+) + NADPH = 7beta-hydroxypregnenolone + NADP(+) - PubMed:17593962
left-to-right
3beta,7alpha-dihydroxyandrost-5-en-17-one + NADP(+) = 3beta- hydroxy-5-androstene-7,17-dione + H(+) + NADPH - PubMed:17593962
left-to-right
3beta-hydroxy-5-androstene-7,17-dione + H(+) + NADPH = 3beta,7beta-dihydroxyandrost-5-en-17-one + NADP(+) - PubMed:17593962
left-to-right
3beta-hydroxy-5alpha-androstane-7,17-dione + H(+) + NADPH = 3beta,7beta-dihydroxy-5alpha-androstan-17-one + NADP(+) - PubMed:17593962
left-to-right