Brand  (β version)

PDB ID: 1T5A

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Ligands
Code Name Style Show Link
OXL Oxalate ion PoSSuM
PO4 Phosphate ion PoSSuM
MG Magnesium ion PoSSuM
K Potassium ion PoSSuM
GOL Glycerol PoSSuM
FBP 1,6-di-O-phosphono-beta-D-fructofuranose PoSSuM
Non-standard Residues
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Structure summary

Code : 1T5A   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Human Pyruvate Kinase M2
Release Data : 2005-07-12
Compound :
mol_id molecule chains
1 Pyruvate kinase, M2 isozyme A,B,C,D
ec: 2.7.1.40
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: PKM2, PKM
expression_system_strain: Rosetta BL21(DE3)pLysS
expression_system_vector_type: plasmid
expression_system_plasmid: pJDAM-His
Authors : Dombrauckas, J.D., Santarsiero, B.D., Mesecar, A.D.
Keywords : alpha8-beta8 barrel, beta sheets, alpha helices, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.8 Å )
Citation :

Structural basis for tumor pyruvate kinase M2 allosteric regulation and catalysis.

Dombrauckas, J.D.,Santarsiero, B.D.,Mesecar, A.D.
(2005)  Biochemistry  44 : 9417 - 9429

PubMed: 15996096
DOI: 10.1021/bi0474923

Reaction

Chain : A, B, C, D
UniProt : P14618 (KPYM_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
[Isoform M2]
pyruvate + ATP = phosphoenolpyruvate + ADP + H(+) 		2.7.1.40 PubMed:15996096, PubMed:1854723, PubMed:20847263
right-to-left
[Isoform M2]
L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+) 		2.7.10.2 PubMed:22306293, PubMed:24120661
left-to-right
[Isoform M2]
L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+) 		2.7.11.1 PubMed:22901803, PubMed:24120661
left-to-right
[Isoform M1]
pyruvate + ATP = phosphoenolpyruvate + ADP + H(+) 		2.7.1.40 PubMed:20847263
-
Cofactor: Evidence: Note:
Mg(2+) ECO:0000305 | PubMed:23530218
-
K(+) ECO:0000305 | PubMed:23530218
-