Brand  (β version)

PDB ID: 1T5A

Hetero Atom Contents

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
FBP Beta-fructose-1,6-diphosphate PoSSuM
GOL Glycerol PoSSuM
K Potassium ion PoSSuM
MG Magnesium ion PoSSuM
OXL Oxalate ion PoSSuM
PO4 Phosphate ion PoSSuM

Structure summary

Code : 1T5A
Header : TRANSFERASE
Title : Human Pyruvate Kinase M2
Release Data : 2005-07-12
Compound :
mol_id molecule chains
1 Pyruvate kinase, M2 isozyme A,B,C,D
ec: 2.7.1.40
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: PKM2, PKM
expression_system_strain: Rosetta BL21(DE3)pLysS
expression_system_vector_type: plasmid
expression_system_plasmid: pJDAM-His
Authors : Dombrauckas, J.D., Santarsiero, B.D., Mesecar, A.D.
Keywords : alpha8-beta8 barrel, beta sheets, alpha helices, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.8 Å )
Citation :

Structural basis for tumor pyruvate kinase M2 allosteric regulation and catalysis.

Dombrauckas, J.D.,Santarsiero, B.D.,Mesecar, A.D.
(2005)  Biochemistry  44 : 9417 - 9429

PubMed: 15996096
DOI: 10.1021/bi0474923

Reaction

Chain : A, B, C, D
UniProt : P14618 (KPYM_HUMAN)
Reaction : ATP + pyruvate = ADP + phosphoenolpyruvate.