Brand  (β version)

PDB ID: 1SEJ

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Ligands
Code Name Style Show Link
UMP 2'-deoxyuridine 5'-monophosphate PoSSuM
F89 S)-2-(5(((1,2-dihydro-3-methyl-1-oxobenzo(f)quinazolin-9-yl)methyl)amino)1-oxo-2-isoindolinyl)glutaric acid PoSSuM
NDP Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate PoSSuM
Non-standard Residues
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Structure summary

Code : 1SEJ   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE, OXIDOREDUCTASE
Title : Crystal Structure of Dihydrofolate Reductase-Thymidylate Synthase from Cryptosporidium hominis Bound to 1843U89/NADPH/dUMP
Release Data : 2004-05-18
Compound :
mol_id molecule chains
1 bifunctional dihydrofolate reductase-thymidylate synthase A,B,C,D,E
ec: 2.1.1.45, 1.5.1.3
Source :
mol_id organism_scientific expression_system
1 Cryptosporidium hominis  (taxid:237895) Escherichia coli  (taxid:562)
expression_system_strain: PA414
Authors : Anderson, A.C.
Keywords : bifunctional enzyme, TRANSFERASE, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 2.87 Å )
Citation :

Two crystal structures of dihydrofolate reductase-thymidylate synthase from Cryptosporidium hominis reveal protein-ligand interactions including a structural basis for observed antifolate resistance.

Anderson, A.C.
(2005)  Acta Crystallogr.,Sect.F  61 : 258 - 262

PubMed: 16511011
DOI: 10.1107/S1744309105002435

Reaction

Chain : A, B, C, D, E
UniProt : Q27552 (Q27552_CRYPV)
Reaction: EC: Evidence:
Physiological Direction:
(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH + H(+) 1.5.1.3 ARBA:ARBA00048873
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dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8- dihydrofolate + dTMP 2.1.1.45 ARBA:ARBA00047344
-