Brand  (β version)

PDB ID: 1DLS

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Ligands
Code Name Style Show Link
MTX Methotrexate PoSSuM
NDP Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate PoSSuM
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Structure summary

Code : 1DLS   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : METHOTREXATE-RESISTANT VARIANTS OF HUMAN DIHYDROFOLATE REDUCTASE WITH SUBSTITUTION OF LEUCINE 22: KINETICS, CRYSTALLOGRAPHY AND POTENTIAL AS SELECTABLE MARKERS
Release Data : 1995-04-20
Compound :
mol_id molecule chains
1 DIHYDROFOLATE REDUCTASE A
ec: 1.5.1.3
Source :
mol_id organism_scientific organism_common
1 Homo sapiens  (taxid:9606) Human
gene: POTENTIAL
Authors : Cody, V., Galitsky, N., Luft, J.R., Pangborn, W.
Keywords : OXIDO-REDUCTASE, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 2.3 Å )
Citation :

Methotrexate-resistant variants of human dihydrofolate reductase with substitutions of leucine 22. Kinetics, crystallography, and potential as selectable markers.

Lewis, W.S.,Cody, V.,Galitsky, N.  et al.
(1995)  J.Biol.Chem.  270 : 5057 - 5064

PubMed: 7890613
DOI: 10.1074/jbc.270.10.5057

Methotrexate-Resistant Variants of Human Dihydrofolate Reductase: Effects of Phe-31 Substitutions

Chunduru, S.K.,Cody, V.,Luft, J.R.  et al.
(1994)  J.Biol.Chem.  269 : 9547

Crystal Structure Determination at 2.3 Angstroms of Recombinant Human Dihydrofolate Reductase Ternary Complex with Nadph and Methotrexate-Gamma-Tetrazole

Cody, V.,Luft, J.R.,Ciszak, E.  et al.
(1992)  Anti-Cancer Drug Des.  7 : 483

Reaction

Chain : A
UniProt : P00374 (DYR_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + H(+) + NADPH 1.5.1.3 PROSITE- ProRule:PRU00660, PubMed:12096917, PubMed:15039552, PubMed:17569517, PubMed:19196009, PubMed:19478082, PubMed:21876184, PubMed:9719595
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