Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
Y1 Yttrium ion
7OA (3s,3ar,5r,7as,8s)-hexahydro-4h-3,5-methanofuro[2,3-B]pyran-8-yl [(2s,3r)-4-[{[2-(cyclopropylamino)-1,3-benzothiazol-6-yl]sulfonyl}(2-methylpropyl)amino]-1-(3,5-difluorophenyl)-3-hydroxybutan-2-yl]carbamate
Code : 6PRF
Header : Hydrolase/Hydrolase Inhibitor
Title : HIV-1 Protease multiple drug resistant clinical isolate mutant PR20 with GRL-14213A
Release Data : 2019-09-18
Compound :
mol_id molecule chains synonym
1 Protease A,B PR, Retropepsin
ec: 3.4.23.16
mutation: Q7K, L10F, I13V, I15V, D30N, V32I, L33F, E35D, M36I, S37N, I47V, I54L, Q58E, I62V, L63P, C67A, A71V, I84V, N88D, L89T, L90M, C95A
Source :
mol_id organism_scientific organism_common expression_system
1 Human immunodeficiency virus type 1 group M subtype  (taxid:11678) HIV-1 Escherichia coli BL21  (taxid:511693)
strain: isolate PR20
gene: gag-pol
Authors : Kneller, D.W., Agniswamy, J., Weber, I.T.
Keywords : Protease, Inhibitor, Multiple Mutant, Viral Protein complex, HYDROLASE, Hydrolase-Hydrolase Inhibitor, Hydrolase-Hydrolase Inhibitor complex
Exp. method : X-RAY DIFFRACTION ( 1.2100 Å )
Citation :

Potent antiviral HIV-1 protease inhibitor combats highly drug resistant mutant PR20.

Kneller, D.W.,Agniswamy, J.,Ghosh, A.K.  et al.
(2019)  Biochem.Biophys.Res.Commun.  519 : 61 - 66

PubMed: 31474336
DOI: 10.1016/j.bbrc.2019.08.126

Chain : A, B
UniProt : P03366 (POL_HV1B1)
Reaction : Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).