Brand  (β version)

PDB ID: 6PRF

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Ligands
Code Name Style Show Link
7OA (3s,3ar,5r,7as,8s)-hexahydro-4h-3,5-methanofuro[2,3-B]pyran-8-yl [(2s,3r)-4-[{[2-(cyclopropylamino)-1,3-benzothiazol-6-yl]sulfonyl}(2-methylpropyl)amino]-1-(3,5-difluorophenyl)-3-hydroxybutan-2-yl]carbamate PoSSuM
Y1 Yttrium ion PoSSuM
Non-standard Residues
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Structure summary

Code : 6PRF   PDBj   RCSB PDB   PDBe
Header : Hydrolase/Hydrolase Inhibitor
Title : HIV-1 Protease multiple drug resistant clinical isolate mutant PR20 with GRL-14213A
Release Data : 2019-09-18
Compound :
mol_id molecule chains synonym
1 Protease A,B PR, Retropepsin
ec: 3.4.23.16
mutation: Q7K, L10F, I13V, I15V, D30N, V32I, L33F, E35D, M36I, S37N, I47V, I54L, Q58E, I62V, L63P, C67A, A71V, I84V, N88D, L89T, L90M, C95A
Source :
mol_id organism_scientific organism_common expression_system
1 Human immunodeficiency virus type 1 group M subtype  (taxid:11678) HIV-1 Escherichia coli BL21  (taxid:511693)
strain: isolate PR20
gene: gag-pol
Authors : Kneller, D.W., Agniswamy, J., Weber, I.T.
Keywords : Protease, Inhibitor, Multiple Mutant, Viral Protein complex, HYDROLASE, Hydrolase-Hydrolase Inhibitor, Hydrolase-Hydrolase Inhibitor complex
Exp. method : X-RAY DIFFRACTION ( 1.2100 Å )
Citation :

Potent antiviral HIV-1 protease inhibitor combats highly drug resistant mutant PR20.

Kneller, D.W.,Agniswamy, J.,Ghosh, A.K.  et al.
(2019)  Biochem.Biophys.Res.Commun.  519 : 61 - 66

PubMed: 31474336
DOI: 10.1016/j.bbrc.2019.08.126

Reaction

Chain : A, B
UniProt : P03366 (POL_HV1B1)
Reaction: EC: Evidence:
Physiological Direction:
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro. 3.4.23.16 PROSITE-ProRule:PRU00275
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3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid. 3.1.13.2 ECO:0000250
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[Reverse transcriptase/ribonuclease H]
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus. 		3.1.26.13 PubMed:2476069
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[Reverse transcriptase/ribonuclease H]
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 		2.7.7.49 PROSITE-ProRule:PRU00405, PubMed:2476069
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[Reverse transcriptase/ribonuclease H]
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 		2.7.7.7 PROSITE-ProRule:PRU00405, PubMed:2476069
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Cofactor: Evidence: Note:
Mg(2+) ECO:0000250
Binds 2 magnesium ions for reverse transcriptase polymerase activity.
Mg(2+) ECO:0000250
Binds 2 magnesium ions for ribonuclease H (RNase H) activity. Substrate-binding is a precondition for magnesium binding.
Mg(2+) ECO:0000250
Magnesium ions are required for integrase activity. Binds at least 1, maybe 2 magnesium ions.