Brand  (β version)

PDB ID: 6O13

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Ligands
Code Name Style Show Link
CKT Cys-ketimine PoSSuM
CL Chloride ion PoSSuM
Non-standard Residues
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Glycosylation
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Modification
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Structure summary

Code : 6O13   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE, LYASE
Title : E. coli cysteine desulfurase SufS H123A with a Cys-ketimine intermediate
Release Data : 2019-07-03
Compound :
mol_id molecule chains synonym
1 Cysteine desulfurase A Selenocysteine beta-lyase,SCL,Selenocysteine lyase,Selenocysteine reductase
ec: 2.8.1.7,4.4.1.16
mutation: H123A
Source :
mol_id organism_scientific expression_system
1 Escherichia coli (strain K12)  (taxid:83333) Escherichia coli BL21(DE3)  (taxid:469008)
strain: K12
gene: sufS, csdB, ynhB, b1680, JW1670
expression_system_vector_type: plasmid
Authors : Dunkle, J.A., Frantom, P.A.
Keywords : cysteine desulfurase, SufS, persulfide, PLP, TRANSFERASE, LYASE
Exp. method : X-RAY DIFFRACTION ( 2.2030 Å )
Citation :

Direct observation of intermediates in the SufS cysteine desulfurase reaction reveals functional roles of conserved active-site residues.

Blahut, M.,Wise, C.E.,Bruno, M.R.  et al.
(2019)  J.Biol.Chem.  294 : 12444 - 12458

PubMed: 31248989
DOI: 10.1074/jbc.RA119.009471

Reaction

Chain : A
UniProt : P77444 (SUFS_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L- alanine 2.8.1.7 PubMed:10329673
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AH2 + L-selenocysteine = A + H(+) + hydrogenselenide + L- alanine 4.4.1.16 PubMed:10329673
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3-sulfino-L-alanine + H2O = H(+) + L-alanine + sulfite - PubMed:10329673
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