Brand  (β version)

PDB ID: 6MHM

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Ligands
Code Name Style Show Link
SO4 Sulfate ion PoSSuM
JRY Hexylcarbamic acid PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
NAG 2-acetamido-2-deoxy-beta-D-glucopyranose
Modification
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Structure summary

Code : 6MHM   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Crystal structure of human acid ceramidase in covalent complex with carmofur
Release Data : 2019-01-23
Compound :
mol_id molecule chains synonym
1 Acid ceramidase subunit alpha A,C Acid CDase,Acylsphingosine deacylase,N-acylsphingosine amidohydrolase,Putative 32 kDa heart protein,PHP32
ec: 3.5.1.23
mol_id molecule chains synonym
2 Acid ceramidase subunit beta B,D Acid CDase,Acylsphingosine deacylase,N-acylsphingosine amidohydrolase,Putative 32 kDa heart protein,PHP32
ec: 3.5.1.23
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Unidentified baculovirus  (taxid:10469)
gene: ASAH1, ASAH, HSD-33, HSD33
mol_id organism_scientific organism_common expression_system
2 Homo sapiens  (taxid:9606) Human Unidentified baculovirus  (taxid:10469)
gene: ASAH1, ASAH, HSD-33, HSD33
Authors : Dementiev, A., Joachimiak, A., Doan, N.
Keywords : acid ceramidase, acid ceramidase inhibitors, benzoxazolone carboxamides, carmofur, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 2.7430 Å )
Citation :

Molecular Mechanism of Inhibition of Acid Ceramidase by Carmofur.

Dementiev, A.,Joachimiak, A.,Nguyen, H.  et al.
(2019)  J. Med. Chem.  62 : 987 - 992

PubMed: 30525581
DOI: 10.1021/acs.jmedchem.8b01723

Reaction

Chain : B, D
UniProt : Q13510 (ASAH1_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
an N-acylsphing-4-enine + H2O = a fatty acid + sphing-4-enine 3.5.1.23 PubMed:10610716, PubMed:11451951, PubMed:12638942, PubMed:12815059, PubMed:15655246, PubMed:22703880, PubMed:27026573, PubMed:29692406, PubMed:7744740, PubMed:8955159
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H2O + N-dodecanoylsphing-4-enine = dodecanoate + sphing-4- enine - PubMed:11451951, PubMed:12764132, PubMed:12815059, PubMed:15655246, PubMed:29692406, PubMed:7744740
right-to-left PubMed:12764132, PubMed:12815059
H2O + N-tetradecanoylsphing-4-enine = sphing-4-enine + tetradecanoate - PubMed:12764132
right-to-left
H2O + N-hexadecanoylsphing-4-enine = hexadecanoate + sphing-4- enine - PubMed:12764132, PubMed:15655246
right-to-left PubMed:12764132
H2O + N-octadecanoylsphing-4-enine = octadecanoate + sphing-4- enine - PubMed:12764132, PubMed:7744740
right-to-left PubMed:12764132
H2O + N-dodecanoyl-(4R)-hydroxysphinganine = (4R)- hydroxysphinganine + dodecanoate - PubMed:12764132
right-to-left
H2O + N-(dodecanoyl)-sphinganine = dodecanoate + sphinganine - PubMed:12764132
right-to-left
H2O + N-(acetyl)-sphing-4-enine = acetate + sphing-4-enine - PubMed:7744740
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H2O + N-(hexanoyl)sphing-4-enine = hexanoate + sphing-4-enine - PubMed:7744740
left-to-right
H2O + N-octanoylsphing-4-enine = octanoate + sphing-4-enine - PubMed:7744740
left-to-right
H2O + N-(9Z-octadecenoyl)-sphing-4-enine = (9Z)-octadecenoate + sphing-4-enine - PubMed:7744740
left-to-right
H2O + N-dodecanoylethanolamine = dodecanoate + ethanolamine - PubMed:15655246
left-to-right