Brand  (β version)

PDB ID: 6MA4

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
JA7 5-{2-[(1r)-2-{(carboxymethyl)[(thiophen-2-yl)methyl]amino}-2-oxo-1-{[(2-oxo-1,2-dihydroquinolin-6-yl)sulfonyl]amino}ethyl]phenoxy}pentanoic acid PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 6MA4   PDBj   RCSB PDB   PDBe
Header : transferase/transferase inhibitor
Title : Crystal structure of human O-GlcNAc transferase bound to a peptide from HCF-1 pro-repeat 2 (11-26) and inhibitor 3a
Release Data : 2018-10-17
Compound :
mol_id molecule chains synonym
1 UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit A O-GlcNAc transferase subunit p110,O-linked N-acetylglucosamine transferase 110 kDa subunit,OGT
ec: 2.4.1.255
mol_id molecule chains
2 Host Cell Factor 1 peptide B
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli BL21(DE3)  (taxid:469008)
gene: OGT
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET24b
mol_id organism_scientific
2 Homo sapiens  (taxid:9606)
synthetic: yes
Authors : Martin, S.E.S., Lazarus, M.B., Walker, S.
Keywords : OGT, O-GlcNAc, glycosyltransferase, enzyme-inhibitor complex, transferase-transferase inhibitor complex
Exp. method : X-RAY DIFFRACTION ( 2.0000 Å )
Citation :

Structure-Based Evolution of Low Nanomolar O-GlcNAc Transferase Inhibitors.

Martin, S.E.S.,Tan, Z.W.,Itkonen, H.M.  et al.
(2018)  J. Am. Chem. Soc.  140 : 13542 - 13545

PubMed: 30285435
DOI: 10.1021/jacs.8b07328

Reaction

Chain : A
UniProt : O15294 (OGT1_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N- acetyl-beta-D-glucosaminyl)-L-seryl-[protein] + H(+) + UDP 2.4.1.255 PubMed:15361863, PubMed:21240259, PubMed:21285374, PubMed:23103939, PubMed:24563466, PubMed:27713473, PubMed:30699359, PubMed:34074792, PubMed:26369908, PubMed:26678539
left-to-right PubMed:24563466, PubMed:27713473, PubMed:30699359, PubMed:34074792
L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O- (N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + H(+) + UDP 2.4.1.255 PubMed:15361863, PubMed:21240259, PubMed:21285374, PubMed:24563466, PubMed:37541260, PubMed:26678539
left-to-right PubMed:24563466, PubMed:27713473, PubMed:37541260
[Isoform 4]
L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N- acetyl-beta-D-glucosaminyl)-L-seryl-[protein] + H(+) + UDP 		2.4.1.255 PubMed:31527085
left-to-right
[Isoform 4]
L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O- (N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + H(+) + UDP 		2.4.1.255 PubMed:31527085
left-to-right