Brand  (β version)

PDB ID: 6HCO

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
FY5 Estrone 3-sulfate PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
NAG 2-acetamido-2-deoxy-beta-D-glucopyranose
Modification
Code Name Show

Structure summary

Code : 6HCO   PDBj   RCSB PDB   PDBe
Header : MEMBRANE PROTEIN
Title : Cryo-EM structure of the ABCG2 E211Q mutant bound to estrone 3-sulfate and 5D3-Fab
Release Data : 2018-11-07
Compound :
mol_id molecule chains synonym
1 ATP-binding cassette sub-family G member 2 A,B Breast cancer resistance protein,CDw338,Mitoxantrone resistance-associated protein,Placenta-specific ATP-binding cassette transporter,Urate exporter
mutation: E211Q
mol_id molecule chains
2 5D3-Fab light chain C,E
mol_id molecule chains
3 5D3-Fab heavy chain D,F
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Homo sapiens  (taxid:9606)
gene: ABCG2, ABCP, BCRP, BCRP1, MXR
expression_system_common: Human
expression_system_cell_line: HEK293 EBNA
mol_id organism_scientific organism_common expression_system
2 Mus musculus  (taxid:10090) House Mouse Mus musculus  (taxid:10090)
expression_system_common: House mouse
expression_system_cell_line: Hybridoma
mol_id organism_scientific organism_common expression_system
3 Mus musculus  (taxid:10090) House Mouse Mus musculus  (taxid:10090)
expression_system_common: House mouse
expression_system_cell_line: Hybridoma
Authors : Manolaridis, I., Jackson, S.M., Taylor, N.M.I., Kowal, J., Stahlberg, H., Locher, K.P.
Keywords : Multidrug transporter, membrane protein, cancer
Exp. method : ELECTRON MICROSCOPY ( 3.58 Å )
Citation :

Cryo-EM structures of a human ABCG2 mutant trapped in ATP-bound and substrate-bound states.

Manolaridis, I.,Jackson, S.M.,Taylor, N.M.I.  et al.
(2018)  Nature  563 : 426 - 430

PubMed: 30405239
DOI: 10.1038/s41586-018-0680-3

Reaction

Chain : A, B
UniProt : Q9UNQ0 (ABCG2_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2. 7.6.2.2 PubMed:11306452, PubMed:12477054, PubMed:12958161, PubMed:31254042
-
ATP + H2O + urate(in) = ADP + H(+) + phosphate + urate(out) - PubMed:36749388, PubMed:19506252, PubMed:20368174, PubMed:22132962, PubMed:31003562
left-to-right PubMed:22132962
ATP + H2O + indoxyl sulfate(in) = ADP + H(+) + indoxyl sulfate(out) + phosphate - UniProtKB:Q7TMS5
left-to-right
ATP + H2O + sphing-4-enine 1-phosphate(in) = ADP + H(+) + phosphate + sphing-4-enine 1-phosphate(out) - PubMed:20110355
left-to-right
ATP + estrone 3-sulfate(in) + H2O = ADP + estrone 3- sulfate(out) + H(+) + phosphate - PubMed:12682043, PubMed:28554189, PubMed:30405239
left-to-right PubMed:12682043
ATP + dehydroepiandrosterone 3-sulfate(in) + H2O = ADP + dehydroepiandrosterone 3-sulfate(out) + H(+) + phosphate - PubMed:12682043
left-to-right
4-methylumbelliferone sulfate(in) + ATP + H2O = 4- methylumbelliferone sulfate(out) + ADP + H(+) + phosphate - PubMed:12682043
left-to-right
5,7-dimethyl-2-methylamino-4-(3-pyridylmethyl)-1,3- benzothiazol-6-yl beta-D-glucuronate(in) + ATP + H2O = 5,7-dimethyl- 2-methylamino-4-(3-pyridylmethyl)-1,3-benzothiazol-6-yl beta-D- glucuronate(out) + ADP + H(+) + phosphate - PubMed:12682043
left-to-right
4-methylumbelliferone beta-D-glucuronate(in) + ATP + H2O = 4- methylumbelliferone beta-D-glucuronate(out) + ADP + H(+) + phosphate - PubMed:12682043
left-to-right
5,7-dimethyl-2-methylamino-4-(3-pyridylmethyl)-1,3- benzothiazol-6-yl sulfate(in) + ATP + H2O = 5,7-dimethyl-2- methylamino-4-(3-pyridylmethyl)-1,3-benzothiazol-6-yl sulfate(out) + ADP + H(+) + phosphate - PubMed:12682043
left-to-right
17beta-estradiol 17-O-(beta-D-glucuronate)(in) + ATP + H2O = 17beta-estradiol 17-O-(beta-D-glucuronate)(out) + ADP + H(+) + phosphate - PubMed:12682043
left-to-right
ATP + H2O + methotrexate(in) = ADP + H(+) + methotrexate(out) + phosphate - PubMed:12682043
left-to-right
ATP + H2O + riboflavin(in) = ADP + H(+) + phosphate + riboflavin(out) - UniProtKB:Q7TMS5
left-to-right
ATP + H2O + pheophorbide a(in) = ADP + H(+) + pheophorbide a(out) + phosphate - UniProtKB:Q7TMS5
left-to-right