Brand  (β version)

PDB ID: 6BZO

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Ligands
Code Name Style Show Link
FI8 Fidaxomicin PoSSuM
MG Magnesium ion PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Glycosylation
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Modification
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Structure summary

Code : 6BZO   PDBj   RCSB PDB   PDBe
Header : transcription/dna/antibiotic
Title : Mtb RNAP Holo/RbpA/Fidaxomicin/upstream fork DNA
Release Data : 2018-03-28
Compound :
mol_id molecule chains synonym
1 DNA-directed RNA polymerase subunit alpha A,B RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
ec: 2.7.7.6
mol_id molecule chains synonym
2 DNA-directed RNA polymerase subunit beta C RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
ec: 2.7.7.6
mol_id molecule chains synonym
3 DNA-directed RNA polymerase subunit beta' D RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
ec: 2.7.7.6
mol_id molecule chains synonym
4 DNA-directed RNA polymerase subunit omega E RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
ec: 2.7.7.6
mol_id molecule chains
5 RNA polymerase sigma factor SigA F
mol_id molecule chains
6 RNA polymerase-binding protein RbpA J
mol_id molecule chains
7 DNA (32-MER) O
mol_id molecule chains
8 DNA (26-MER) P
Source :
mol_id organism_scientific expression_system
1 Mycobacterium tuberculosis  (taxid:1773) Escherichia coli  (taxid:562)
gene: rpoA, rpoA_1, CLD25_18970, ERS007661_01801
mol_id organism_scientific expression_system
2 Mycobacterium tuberculosis  (taxid:1773) Escherichia coli  (taxid:562)
gene: rpoB, SAMEA2682864_01701
mol_id organism_scientific expression_system
3 Mycobacterium tuberculosis  (taxid:1773) Escherichia coli  (taxid:562)
gene: rpoC, rpoC_1, CLD25_03785
mol_id organism_scientific expression_system
4 Mycobacterium tuberculosis  (taxid:1773) Escherichia coli  (taxid:562)
gene: rpoZ
mol_id organism_scientific expression_system
5 Mycobacterium tuberculosis  (taxid:1773) Escherichia coli  (taxid:562)
gene: sigA, hrdB_2, CLD25_14885
mol_id organism_scientific expression_system
6 Mycobacterium tuberculosis  (taxid:1773) Escherichia coli  (taxid:562)
gene: rbpA, CLD25_11245
mol_id organism_scientific
7 Mycobacterium tuberculosis  (taxid:1773)
synthetic: yes
mol_id organism_scientific
8 Mycobacterium tuberculosis  (taxid:1773)
synthetic: yes
Authors : Darst, S.A., Campbell, E.A., Boyaci Selcuk, H., Chen, J.
Keywords : RNA Polymerase, Antibiotic, Inhibitor, TRANSCRIPTION, transcription-dna-antibiotic complex
Exp. method : ELECTRON MICROSCOPY ( 3.38 Å )
Citation :

Fidaxomicin jamsMycobacterium tuberculosisRNA polymerase motions needed for initiation via RbpA contacts.

Boyaci, H.,Chen, J.,Lilic, M.  et al.
(2018)  Elife  7

PubMed: 29480804
DOI: 10.7554/eLife.34823

Reaction

Chain : A, B
UniProt : A0A045J8T1 (A0A045J8T1_MYCTX)
Reaction: EC: Evidence:
Physiological Direction:
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate 2.7.7.6 ARBA:ARBA00048552, HAMAP-Rule:MF_00059
-
Chain : C
UniProt : V9Z879 (V9Z879_MYCTX)
Reaction: EC: Evidence:
Physiological Direction:
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate 2.7.7.6 ARBA:ARBA00048552, HAMAP-Rule:MF_01321, RuleBase:RU363031
-
Chain : D
UniProt : A0A045J9E2 (A0A045J9E2_MYCTX)
Reaction: EC: Evidence:
Physiological Direction:
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate 2.7.7.6 ARBA:ARBA00048552, HAMAP-Rule:MF_01322, RuleBase:RU004279
-
Cofactor: Evidence: Note:
Mg(2+) ECO:0000256 | HAMAP-Rule:MF_01322
Binds 1 Mg(2+) ion per subunit.
Zn(2+) ECO:0000256 | HAMAP-Rule:MF_01322
Binds 2 Zn(2+) ions per subunit.
Chain : E
UniProt : A0A0T9N9K3 (A0A0T9N9K3_MYCTX)
Reaction: EC: Evidence:
Physiological Direction:
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate 2.7.7.6 ARBA:ARBA00048552, HAMAP-Rule:MF_00366
-
Chain : F
UniProt : A0A045HD00 (A0A045HD00_MYCTX)
Reaction : -
Chain : J
UniProt : A0A045IP01 (A0A045IP01_MYCTX)
Reaction : -