Brand  (β version)

PDB ID: 6AAZ

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Ligands
Code Name Style Show Link
9UC N6-{[(4-nitrophenyl)methoxy]carbonyl}-L-lysine PoSSuM
ATP Adenosine-5'-triphosphate PoSSuM
CIT Citric acid PoSSuM
K Potassium ion PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
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Structure summary

Code : 6AAZ   PDBj   RCSB PDB   PDBe
Header : TRANSLATION
Title : Crystal structure of Methanosarcina mazei PylRS(Y306A/Y384F) complexed with pNO2ZLys
Release Data : 2019-04-17
Compound :
mol_id molecule chains synonym
1 Pyrrolysine--tRNA ligase A Pyrrolysyl-tRNA synthetase,PylRS
ec: 6.1.1.26
mutation: Y306A, Y384F
Source :
mol_id organism_scientific expression_system
1 Methanosarcina mazei JCM 9314  (taxid:1293038) Escherichia coli BL21(DE3)  (taxid:469008)
gene: pylS
expression_system_variant: rosetta
Authors : Yanagisawa, T., Kuratani, M., Yokoyama, S.
Keywords : aminoacyl-tRNA synthetase, non-natural amino acids, TRANSLATION
Exp. method : X-RAY DIFFRACTION ( 1.842 Å )
Citation :

Structural Basis for Genetic-Code Expansion with Bulky Lysine Derivatives by an Engineered Pyrrolysyl-tRNA Synthetase.

Yanagisawa, T.,Kuratani, M.,Seki, E.  et al.
(2019)  Cell Chem Biol  26 : 936

PubMed: 31031143
DOI: 10.1016/j.chembiol.2019.03.008

Reaction

Chain : A
UniProt : A0A0F8JXW8 (A0A0F8JXW8_METMZ)
Reaction: EC: Evidence:
Physiological Direction:
tRNA(Pyl) + L-pyrrolysine + ATP = L-pyrrolysyl-tRNA(Pyl) + AMP + diphosphate 6.1.1.26 HAMAP-Rule:MF_01573
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