Brand  (β version)

PDB ID: 6AAW

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
Non-standard Residues
Code Name Show
0EH (2r)-2-amino-2-methylnonanoic acid
2JH 3-cyclobutyl-L-alanine
DTR D-tryptophan
MK8 2-methyl-L-norleucine
Glycosylation
Code Name Emphasize
Modification
Code Name Show
ACE Acetyl group
NH2 Amino group

Structure summary

Code : 6AAW   PDBj   RCSB PDB   PDBe
Header : LIGASE
Title : Mdm2 in complex with a D amino Acid Containing Stapled Peptide
Release Data : 2019-07-24
Compound :
mol_id molecule chains synonym
1 E3 ubiquitin-protein ligase Mdm2 A Double minute 2 protein,Hdm2,Oncoprotein Mdm2,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2
ec: 2.3.2.27
fragment: UNP residues 6-125
mol_id molecule chains
2 ACE-LEU-THR-PHE-STQ-GLU-TYR-DTR-GLN-LEU-CBA-MK8-SER-ALA-ALA B
other_details: Chemically synthesized Helically Stabilized Peptide Designed to disrupt the Mdm2/p53 protein:protein interaction. Template sequence before incorporation of non natural amino acids was derived from M13 phage display.
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli BL21  (taxid:511693)
gene: MDM2
expression_system_strain: BL21
mol_id organism_scientific
2 Enterobacteria phage M13  (taxid:10870)
synthetic: yes
Authors : Brown, C.J., Partridge, A.W.
Keywords : E3 ligase, LIGASE
Exp. method : X-RAY DIFFRACTION ( 2.00 Å )
Citation :

A Model System to Explore Macrocyclic Peptide Structural and Chirality Relationships: Tolerance of helix-breaking residues within the context of a stapled peptide.

Partridge, A.W.,Hung, Y.K.K.,Juang, Y.  et al.
To Be Published 

Reaction

Chain : A
UniProt : Q00987 (MDM2_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. 2.3.2.27 PubMed:12821780
-