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Ligands
Code Name Style Show Link
9RL (2r,3s)-4-(4-chlorophenyl)-2-phenyl-3-(1h-1,2,4-triazol-1-yl)butan-2-ol
CL Chloride ion
GOL Glycerol
HEM Protoporphyrin ix containing Fe
Non-standard Residues
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Code : 6A17   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Crystal structure of CYP90B1 in complex with brassinazole
Release Data : 2019-06-12
Compound :
mol_id molecule chains synonym
1 Cytochrome P450 90B1 A Protein DWARF 4,Dwarf4,Steroid 22-alpha-hydroxylase
ec: 1.14.-.-
mutation: M1-L28 was substituted with MA. E256-V277 and N435-S446 were deleted, P506L
Source :
mol_id organism_scientific organism_common expression_system
1 Arabidopsis thaliana  (taxid:3702) Mouse-ear cress Escherichia coli BL21(DE3)  (taxid:469008)
gene: CYP90B1, DWF4, At3g50660, T3A5.40
expression_system_strain: BL21 (DE3)
Authors : Fujiyama, K., Hino, T., Kanadani, M., Mizutani, M., Nagano, S.
Keywords : cytochrome P450, phytohormone, brassinosteroid, brassinazole, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 2.301 Å )
Citation :

Structural insights into a key step of brassinosteroid biosynthesis and its inhibition.

Fujiyama, K.,Hino, T.,Kanadani, M.  et al.
(2019)  Nat.Plants  5 : 589 - 594

PubMed: 31182839
DOI: 10.1038/s41477-019-0436-6

Chain : A
UniProt : O64989 (C90B1_ARATH)
Reaction: EC: Evidence:
Physiological Direction:
a C27-steroid + O2 + reduced [NADPH--hemoprotein reductase] = a (22S)-22-hydroxy C27-steroid + H(+) + H2O + oxidized [NADPH-- hemoprotein reductase] 1.14.14.178 PubMed:16460510
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a C28-steroid + O2 + reduced [NADPH--hemoprotein reductase] = a (22S)-22-hydroxy C28-steroid + H(+) + H2O + oxidized [NADPH-- hemoprotein reductase] 1.14.14.178 PubMed:16460510, PubMed:9490746
left-to-right
a C29-steroid + O2 + reduced [NADPH--hemoprotein reductase] = a (22S)-22-hydroxy C29-steroid + H(+) + H2O + oxidized [NADPH-- hemoprotein reductase] 1.14.14.178 PubMed:16460510
left-to-right
cholesterol + O2 + reduced [NADPH--hemoprotein reductase] = (22S)-22-hydroxycholesterol + H(+) + H2O + oxidized [NADPH-- hemoprotein reductase] - PubMed:16460510
left-to-right
cholestanol + O2 + reduced [NADPH--hemoprotein reductase] = (22S)-22-hydroxycholestanol + H(+) + H2O + oxidized [NADPH-- hemoprotein reductase] - PubMed:16460510
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campestanol + O2 + reduced [NADPH--hemoprotein reductase] = 6- deoxycathasterone + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:16460510, PubMed:9490746
left-to-right PubMed:16460510, PubMed:9490746
campesterol + O2 + reduced [NADPH--hemoprotein reductase] = (22S)-22-hydroxycampesterol + H(+) + H2O + oxidized [NADPH-- hemoprotein reductase] - PubMed:16460510
left-to-right
6-oxocampestanol + O2 + reduced [NADPH--hemoprotein reductase] = cathasterone + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:16460510, PubMed:9490746
left-to-right PubMed:16460510, PubMed:9490746
O2 + reduced [NADPH--hemoprotein reductase] + sitosterol = (22S)-22-hydroxysitosterol + H(+) + H2O + oxidized [NADPH-- hemoprotein reductase] - PubMed:16460510
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