Brand  (β version)

PDB ID: 6S83

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Ligands
Code Name Style Show Link
MDN Methylenediphosphonic acid PoSSuM
MG Magnesium ion PoSSuM
SAM S-adenosylmethionine PoSSuM
PO4 Phosphate ion PoSSuM
ACP Phosphomethylphosphonic acid adenylate ester PoSSuM
Non-standard Residues
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Structure summary

Code : 6S83   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal structure of methionine adenosyltransferase from Pyrococcus furiosus in complex with AMPPCP, SAM, and PCP
Release Data : 2020-02-05
Compound :
mol_id molecule chains synonym
1 S-adenosylmethionine synthase A,B,C,D,E,F,G,H AdoMet synthase,Methionine adenosyltransferase
ec: 2.5.1.6
Source :
mol_id organism_scientific expression_system
1 Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)  (taxid:186497) Escherichia coli BL21(DE3)  (taxid:469008)
strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1
gene: mat, PF1866
expression_system_strain: BL21(DE3)
expression_system_vector_type: Plasmid
expression_system_plasmid: pET22b
Authors : Degano, M., Minici, C., Porcelli, M.
Keywords : S-adenosyl methionine synthesis, cofactor biosynthesis, transferase, cytoplasmic
Exp. method : X-RAY DIFFRACTION ( 2.3360 Å )
Citation :

Structures of catalytic cycle intermediates of the Pyrococcus furiosus methionine adenosyltransferase demonstrate negative cooperativity in the archaeal orthologues.

Minici, C.,Mosca, L.,Ilisso, C.P.  et al.
(2020)  J.Struct.Biol.  210 : 107462 - 107462

PubMed: 31962159
DOI: 10.1016/j.jsb.2020.107462

Reaction

Chain : A, B, C, D, E, F, G, H
UniProt : Q8TZW1 (METK_PYRFU)
Reaction: EC: Evidence:
Physiological Direction:
L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate 2.5.1.6 HAMAP-Rule:MF_00136
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Cofactor: Evidence: Note:
Mg(2+) ECO:0000255 | HAMAP-Rule:MF_00136
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