Brand  (β version)

PDB ID: 6RKW

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Ligands
Code Name Style Show Link
ANP Phosphoaminophosphonic acid-adenylate ester PoSSuM
JHN (3~{R})-3-[[4-(3,4-dihydro-2~{H}-pyrano[2,3-C]pyridin-6-ylmethylamino)piperidin-1-yl]methyl]-1,4,7-triazatricyclo[6.3.1.0^{4,12}]dodeca-6,8(12),9-triene-5,11-dione PoSSuM
Non-standard Residues
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Structure summary

Code : 6RKW   PDBj   RCSB PDB   PDBe
Header : ISOMERASE
Title : CryoEM structure of the complete E. coli DNA Gyrase complex bound to a 130 bp DNA duplex
Release Data : 2019-11-06
Compound :
mol_id molecule chains
1 DNA gyrase subunit A A,C
ec: 5.6.2.3
other_details: GyrA subunit
mol_id molecule chains synonym
2 DNA gyrase subunit B B,D Type IIA topoisomerase subunit GyrB
ec: 5.6.2.3
other_details: GyrB subunit
mol_id molecule chains
3 DNA (58-MER) F,E
mol_id molecule chains
4 DNA (62-MER) G,H
Source :
mol_id organism_scientific expression_system
1 Escherichia coli (strain K12)  (taxid:83333) Escherichia coli BL21(DE3)  (taxid:469008)
gene: gyrA, hisW, nalA, parD, b2231, JW2225
mol_id organism_scientific expression_system
2 Escherichia coli (strain K12)  (taxid:83333) Escherichia coli BL21  (taxid:511693)
gene: gyrB, acrB, cou, himB, hisU, nalC, parA, pcbA, b3699, JW5625
mol_id organism_scientific
3 Escherichia coli  (taxid:562)
synthetic: yes
mol_id organism_scientific
4 Escherichia coli  (taxid:562)
synthetic: yes
Authors : Vanden Broeck, A., Lamour, V.
Keywords : Isomerase, Complex, DNA Gyrase, Inhibitor, DNA BINDING PROTEIN
Exp. method : ELECTRON MICROSCOPY ( 6.6 Å )
Citation :

Cryo-EM structure of the complete E. coli DNA gyrase nucleoprotein complex.

Vanden Broeck, A.,Lotz, C.,Ortiz, J.  et al.
(2019)  Nat Commun  10 : 4935 - 4935

PubMed: 31666516
DOI: 10.1038/s41467-019-12914-y

Reaction

Chain : A, C
UniProt : P0AES4 (GYRA_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
ATP-dependent breakage, passage and rejoining of double- stranded DNA. 5.6.2.2 HAMAP-Rule:MF_01897, PubMed:12051842, PubMed:18642932, PubMed:186775, PubMed:19965760, PubMed:9278055
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Chain : B, D
UniProt : P0AES6 (GYRB_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
ATP-dependent breakage, passage and rejoining of double- stranded DNA. 5.6.2.2 HAMAP-Rule:MF_01898, PubMed:12051842, PubMed:12051843, PubMed:18642932, PubMed:186775, PubMed:19965760, PubMed:20675723, PubMed:22731783
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