Brand  (β version)

PDB ID: 6PZ3

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Ligands
Code Name Style Show Link
GOL Glycerol PoSSuM
MG Magnesium ion PoSSuM
XG4 2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]guanosine PoSSuM
Non-standard Residues
Code Name Show
CAR Cytosine arabinose-5'-phosphate
Glycosylation
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Modification
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Structure summary

Code : 6PZ3   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Polymerase Eta-catalyzed insertion of correct G opposite template cytarabine (AraC) residue
Release Data : 2019-11-20
Compound :
mol_id molecule chains synonym
1 DNA polymerase eta A RAD30 homolog A,Xeroderma pigmentosum variant type protein
ec: 2.7.7.7
mutation: M406C
mol_id molecule chains
2 DNA Primer strand P
mol_id molecule chains
3 DNA template containing cytarabine (AraC) residue T
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli BL21(DE3)  (taxid:469008)
gene: POLH, RAD30, RAD30A, XPV
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: PET28
mol_id organism_scientific
2 Synthetic construct  (taxid:32630)
synthetic: yes
mol_id organism_scientific
3 Synthetic construct  (taxid:32630)
synthetic: yes
Authors : Rechkoblit, O., Aggarwal, A.K.
Keywords : cytarabin, lesion bypass, DNA damage, replication, chemotherapy, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.3950 Å )
Citation :

Structural insights into mutagenicity of anticancer nucleoside analog cytarabine during replication by DNA polymerase eta.

Rechkoblit, O.,Johnson, R.E.,Buku, A.  et al.
(2019)  Sci Rep  9 : 16400 - 16400

PubMed: 31704958
DOI: 10.1038/s41598-019-52703-7

Reaction

Chain : A
UniProt : Q9Y253 (POLH_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.7 PubMed:27284197
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