Brand  (β version)

PDB ID: 6MI1

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Ligands
Code Name Style Show Link
GOL Glycerol PoSSuM
SO4 Sulfate ion PoSSuM
BGC Beta-D-glucopyranose PoSSuM
U1Y Methyl 6-thio-beta-D-glucopyranoside PoSSuM
: Polysaccharide
Non-standard Residues
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Glycosylation
Code Name Emphasize
NAG 2-acetamido-2-deoxy-beta-D-glucopyranose
Modification
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Structure summary

Code : 6MI1   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : CRYSTAL STRUCTURE ANALYSIS OF THE VARIANT PLANT EXOHYDROLASE ARG158ALA-GLU161ALA IN COMPLEX WITH METHYL 6-THIO-BETA-GENTIOBIOSIDE
Release Data : 2019-05-29
Compound :
mol_id molecule chains
1 Beta-D-glucan exohydrolase isoenzyme ExoI A
fragment: UNP RESIDUES 26-627
mutation: ARG158ALA, GLU161ALA
Source :
mol_id organism_scientific organism_common expression_system
1 Hordeum vulgare subsp. vulgare  (taxid:112509) Domesticated barley Komagataella pastoris  (taxid:4922)
expression_system_strain: SMD11680H
expression_system_vector_type: PLASMID
expression_system_plasmid: PPICZALPHABNH8/DEST
Authors : Streltsov, V.A., Luang, S., Hrmova, M.
Keywords : BETA BARREL, HYDROLASE, GRAIN DEVELOPMENT, TIM BARREL/BETA SHEET, N-GLYCOSYLATION, PLANT APOPLAST
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

Discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site.

Streltsov, V.A.,Luang, S.,Peisley, A.  et al.
(2019)  Nat Commun  10 : 2222 - 2222

PubMed: 31110237
DOI: 10.1038/s41467-019-09691-z

Reaction

Chain : A
UniProt : Q9XEI3 (Q9XEI3_HORVV)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose. 3.2.1.21 ARBA:ARBA00000448
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