Brand  (β version)

PDB ID: 6M7P

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Ligands
Code Name Style Show Link
DZ4 2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]adenosine PoSSuM
GOL Glycerol PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
Code Name Show
02I (6s,7s,8s,10r)-4-amino-8-hydroxy-7,8,9,10-tetrahydro-6h-7,10-epoxyazepino[1,2-E]purin-6-yl dihydrogen phosphate
Glycosylation
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Modification
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Structure summary

Code : 6M7P   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/DNA
Title : Human DNA polymerase eta extension complex with cdA at the -2 position
Release Data : 2018-09-12
Compound :
mol_id molecule chains synonym
1 DNA polymerase eta A RAD30 homolog A,Xeroderma pigmentosum variant type protein
ec: 2.7.7.7
fragment: residues 1-432
mol_id molecule chains
2 DNA (5'-D(*CP*AP*TP*TP*CP*(02I)P*CP*AP*CP*AP*CP*T)-3') T
mol_id molecule chains
3 DNA (5'-D(*AP*GP*TP*GP*TP*GP*TP*G)-3') P
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: POLH, RAD30, RAD30A, XPV
mol_id organism_scientific organism_common
2 Homo sapiens  (taxid:9606) Human
synthetic: yes
mol_id organism_scientific organism_common
3 Homo sapiens  (taxid:9606) Human
synthetic: yes
Authors : Gregory, M.T., Yang, W.
Keywords : TRANSLESION SYNTHESIS, cdA, DNA BINDING PROTEIN, TRANSFERASE-DNA complex
Exp. method : X-RAY DIFFRACTION ( 1.7500 Å )
Citation :

Bypassing a 8,5'-cyclo-2'-deoxyadenosine lesion by human DNA polymerase eta at atomic resolution.

Weng, P.J.,Gao, Y.,Gregory, M.T.  et al.
(2018)  Proc. Natl. Acad. Sci. U.S.A.  115 : 10660 - 10665

PubMed: 30275308
DOI: 10.1073/pnas.1812856115

Reaction

Chain : A
UniProt : Q9Y253 (POLH_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.7 PubMed:27284197, PubMed:38212351
-
Cofactor: Evidence: Note:
Mn(2+) ECO:0000269 | PubMed:27284197
ECO:0000269 | PubMed:27284197
Binds 2 Mg(2+) (PubMed:27284197). Prefers Mg(2+), but can also use Mn(2+) (PubMed:27284197). In vitro, can also utilize other divalent cations such as Ca(2+) (PubMed:27284197).