Brand  (β version)

PDB ID: 6M7J

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Ligands
Code Name Style Show Link
C0L Methyl [(1e,5r)-5-{(3e)-3-[(2e,4e,8r,9e,12e)-1,8-dihydroxy-2,5,9-trimethyltetradeca-2,4,9,12-tetraen-1-ylidene]-2,4-dioxo-3,4-D ihydro-2h-pyran-6-yl}hex-1-en-1-yl]carbamate PoSSuM
MG Magnesium ion PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 6M7J   PDBj   RCSB PDB   PDBe
Header : TRANSCRIPTION
Title : Mycobacterium tuberculosis RNAP with RbpA/us fork and Corallopyronin
Release Data : 2018-11-21
Compound :
mol_id molecule chains synonym
1 DNA-directed RNA polymerase subunit alpha A,B RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
ec: 2.7.7.6
mol_id molecule chains synonym
2 DNA-directed RNA polymerase subunit beta C RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
ec: 2.7.7.6
mol_id molecule chains synonym
3 DNA-directed RNA polymerase subunit beta' D RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
ec: 2.7.7.6
mol_id molecule chains synonym
4 DNA-directed RNA polymerase subunit omega E RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
ec: 2.7.7.6
mol_id molecule chains synonym
5 RNA polymerase sigma factor SigA F Sigma-A
mol_id molecule chains
6 RNA polymerase-binding protein RbpA J
mol_id molecule chains
7 DNA (31-MER) O
mol_id molecule chains
8 DNA (26-MER) P
Source :
mol_id organism_scientific expression_system
1 Mycobacterium tuberculosis  (taxid:1773) Escherichia coli  (taxid:562)
gene: rpoA
mol_id organism_scientific expression_system
2 Mycobacterium tuberculosis  (taxid:1773) Escherichia coli  (taxid:562)
gene: rpoB, SAMEA2682864_01701
mol_id organism_scientific expression_system
3 Mycobacterium tuberculosis  (taxid:1773) Escherichia coli  (taxid:562)
gene: rpoC
mol_id organism_scientific expression_system
4 Mycobacterium tuberculosis  (taxid:1773) Escherichia coli  (taxid:562)
gene: rpoZ, ERS007672_03979, ERS007703_04032, ERS007720_04749, ERS027652_00548, ERS027654_02543, ERS027656_03959, ERS124361_02246
mol_id organism_scientific expression_system
5 Mycobacterium tuberculosis  (taxid:1773) Escherichia coli  (taxid:562)
gene: sigA, mysA, rpoD, rpoV
mol_id organism_scientific expression_system
6 Mycobacterium tuberculosis  (taxid:1773) Escherichia coli  (taxid:562)
gene: rbpA
mol_id organism_scientific
7 Synthetic construct  (taxid:32630)
synthetic: yes
mol_id organism_scientific
8 Synthetic construct  (taxid:32630)
synthetic: yes
Authors : Darst, S.A., Campbell, E.A., Boyaci Selcuk, H., Chen, J.
Keywords : initiation, transcription bubble, closed clamp, open promoter complex, TRANSCRIPTION, TRANSCRIPTION-DNA complex
Exp. method : ELECTRON MICROSCOPY ( 4.4 Å )
Citation :

Structures of an RNA polymerase promoter melting intermediate elucidate DNA unwinding.

Boyaci, H.,Chen, J.,Jansen, R.  et al.
(2019)  Nature  565 : 382 - 385

PubMed: 30626968
DOI: 10.1038/s41586-018-0840-5

Reaction

Chain : A, B
UniProt : A5U8D3 (RPOA_MYCTA)
Reaction: EC: Evidence:
Physiological Direction:
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate 2.7.7.6 HAMAP-Rule:MF_00059
-
Chain : C
UniProt : V9Z879 (V9Z879_MYCTX)
Reaction: EC: Evidence:
Physiological Direction:
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate 2.7.7.6 ARBA:ARBA00048552, HAMAP-Rule:MF_01321, RuleBase:RU363031
-
Chain : D
UniProt : A5U053 (RPOC_MYCTA)
Reaction: EC: Evidence:
Physiological Direction:
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate 2.7.7.6 HAMAP-Rule:MF_01322
-
Cofactor: Evidence: Note:
Mg(2+) ECO:0000255 | HAMAP-Rule:MF_01322
Binds 1 Mg(2+) ion per subunit.
Zn(2+) ECO:0000255 | HAMAP-Rule:MF_01322
Binds 2 Zn(2+) ions per subunit.
Chain : E
UniProt : A0A0T9N9K3 (A0A0T9N9K3_MYCTX)
Reaction: EC: Evidence:
Physiological Direction:
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate 2.7.7.6 ARBA:ARBA00048552, HAMAP-Rule:MF_00366
-
Chain : F
UniProt : P9WGI0 (SIGA_MYCTO)
Reaction : -
Chain : J
UniProt : P9WHJ4 (RBPA_MYCTO)
Reaction : -