Brand  (β version)

PDB ID: 6JCZ

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Ligands
Code Name Style Show Link
9TY Cyclopropane-1,1-dicarboxylic acid PoSSuM
MG Magnesium ion PoSSuM
NDP Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate PoSSuM
Non-standard Residues
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Structure summary

Code : 6JCZ   PDBj   RCSB PDB   PDBe
Header : ISOMERASE
Title : Cryo-EM Structure of Sulfolobus solfataricus ketol-acid reductoisomerase (Sso-KARI) in complex with Mg2+, NADPH, and CPD at pH7.5
Release Data : 2019-04-17
Compound :
mol_id molecule chains synonym
1 Putative ketol-acid reductoisomerase 2 A,B,C,D,E,F,G,H,I,J,K,L Acetohydroxy-acid isomeroreductase 2,Alpha-keto-beta-hydroxylacyl reductoisomerase 2
ec: 1.1.1.86
Source :
mol_id organism_scientific organism_common expression_system
1 Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)  (taxid:273057) Sulfolobus solfataricus Escherichia coli BL21(DE3)  (taxid:469008)
strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2
gene: ilvC2, ilvC-2, SSO1322
expression_system_strain: BL21(DE3)
Authors : Chen, C.Y., Chang, Y.C., Lin, K.F., Huang, C.H., Lin, B.L., Ko, T.P., Hsieh, D.L., Tsai, M.D.
Keywords : Bi-specific, Thermostable, Reductoisomerase, Magnesium-dependent, Dodecamer, Knotted protein, ISOMERASE
Exp. method : ELECTRON MICROSCOPY ( 3.35 Å )
Citation :

Use of Cryo-EM To Uncover Structural Bases of pH Effect and Cofactor Bispecificity of Ketol-Acid Reductoisomerase.

Chen, C.Y.,Chang, Y.C.,Lin, B.L.  et al.
(2019)  J. Am. Chem. Soc.  141 : 6136 - 6140

PubMed: 30921515
DOI: 10.1021/jacs.9b01354

Reaction

Chain : A, B, C, D, E, F, G, H, I, J, K, L
UniProt : Q97YJ9 (ILVC2_SACS2)
Reaction: EC: Evidence:
Physiological Direction:
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2- acetolactate + H(+) + NADPH 1.1.1.86 -
-
(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2- ethyl-2-hydroxy-3-oxobutanoate + H(+) + NADPH 1.1.1.86 -
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