Brand  (β version)

PDB ID: 6HUY

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Ligands
Code Name Style Show Link
FE9 Iron-guanylyl pyridinol cofactor PoSSuM
GOL Glycerol PoSSuM
GUE 5,10-methenyltetrahydrofolate PoSSuM
DMS Dimethyl sulfoxide PoSSuM
PG4 Tetraethylene glycol PoSSuM
Non-standard Residues
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CSO S-hydroxycysteine
Glycosylation
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Structure summary

Code : 6HUY   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : HmdII from Desulfurobacterium thermolithotrophum reconstitued with Fe-guanylylpyridinol (FeGP) cofactor and co-crystallized with methenyl-tetrahydrofolate form A
Release Data : 2019-01-09
Compound :
mol_id molecule chains
1 Coenzyme F420-dependent N(5),N(10)-methenyltetrahydromethanopterin reductase-related protein A,C
ec: 1.12.98.2
other_details: The poly-histidine tag is attached on the C-terminal.
mol_id molecule chains
2 Coenzyme F420-dependent N(5),N(10)-methenyltetrahydromethanopterin reductase-related protein B,D
other_details: The poly-histidine tag is attached on the C-terminal. From the electron density, the Cys117 seems to be oxidized (CSO).
Source :
mol_id organism_scientific expression_system
1 Desulfurobacterium thermolithotrophum DSM 11699  (taxid:868864) Escherichia coli BL21(DE3)  (taxid:469008)
cell_line: /
atcc: /
organ: /
tissue: /
cell: /
gene: Dester_1504
expression_system_atcc_number: /
expression_system_organ: /
expression_system_tissue: /
expression_system_cell: /
expression_system_vector_type: Plasmid
expression_system_vector: pET-24b(+)
mol_id organism_scientific expression_system
2 Desulfurobacterium thermolithotrophum DSM 11699  (taxid:868864) Escherichia coli BL21(DE3)  (taxid:469008)
cell_line: /
atcc: /
organ: /
tissue: /
cell: /
gene: Dester_1504
expression_system_atcc_number: /
expression_system_organ: /
expression_system_tissue: /
expression_system_cell: /
expression_system_vector_type: Plasmid
expression_system_vector: pET-24b(+)
Authors : Watanabe, T., Wagner, T., Huang, G., Kahnt, J., Ataka, K., Ermler, U., Shima, S.
Keywords : Hydrogenase, H2-activation, Lateral gene-transfer, cofactor biosynthesis, tetrahydromethanopterin, tetrahydrofolate, paralog, sulfur-reducing bacteria, metalloenzyme, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 2.250 Å )
Citation :

The Bacterial [Fe]-Hydrogenase Paralog HmdII Uses Tetrahydrofolate Derivatives as Substrates.

Watanabe, T.,Wagner, T.,Huang, G.  et al.
(2019)  Angew. Chem. Int. Ed. Engl.  58 : 3506 - 3510

PubMed: 30600878
DOI: 10.1002/anie.201813465

Reaction

Chain : B, D
UniProt : F0S2B6 (F0S2B6_DESTD)
Reaction : -